Artificial Intelligence and Network Medicine: Path to Precision Medicine.

Over the past two decades, network medicine (NM) has evolved to help define disease mechanisms, identify drug targets, and guide increasingly precise therapies. In recent years, the integration of NM with artificial intelligence (AI), particularly deep learning techniques, has evolved with increasing applications. AI techniques help elucidate complex disease mechanisms and define precise therapies.

Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance.

The presentation of peptides on HLA molecules is essential to CD8 T cell responses. Here, we show that loss of uL14 significantly downregulates the expression of antigen processing and presentation (APP) components in melanoma cell lines. Peptides generated following knockdown show different characteristics, with altered peptide charge, and differences in anchor residue positions.

3D-Printed Microfiltration Membranes via Dual-Wavelength Microstereolithography.

A new and sustainable membrane manufacturing method is 3D printing, which reduces the number of fabrication steps, waste production, and the corresponding CO emissions. It further enables fabricating membranes with well-defined pore size, shape, and configuration. Here, we study 3D printing of microfiltration membranes using a novel dual-wavelength microstereolithography method.

Association of physical activity level during pregnancy with preterm birth and infant adiposity: longitudinal analysis from the MAASTHI cohort, India.

Background: Physical activity during pregnancy is thought to influence birth outcomes, but its association with it is not fully understood. We aimed to examine the association of sedentary behavior during pregnancy with preterm birth and infant adiposity measured at birth, 14 weeks, and one year of age.

Methods: In this cohort study, physical activity during pregnancy was assessed using the physical activity questionnaire.

and reciprocally promote their virulence factor secretion and pro-inflammatory effects.

Background: Co-infections of and can significantly increase morbidity and mortality. However, the effect of co-existence on virulence factor secretion and pro-inflammatory effects remain elusive.

Methods: We systematically investigated the virulence factors released by and under different culturing conditions using proteomics.

Growth of High Aspect Ratio Wurtzite GaAs Nanowires.

Crystal phase control of III-V semiconductor nanowires grown by the vapor liquid solid mechanism has emerged as a new frontier in nanomaterials in the 2010s. Of particular interest is the ability to grow the metastable wurtzite crystal, which is commercially unavailable in semiconductors such as GaAs and SiGe. The successful growth of wurtzite GaAs nanowires has been demonstrated by precise control of the wetting contact angle of the catalyst particle.

Glycoproteomics analysis of complement factor H and its complement-regulatory function during -associated hemolytic uremic syndrome.

Hemolytic uremic syndrome caused by an invasive infection (SP-HUS) is a rare and severe disease that primarily affects children under two years of age. The pathophysiology of SP-HUS remains poorly understood, and treatment is largely supportive. Complement factor H (FH) is a key regulator of the alternative pathway of the complement system.

A genome-wide association study identifies new loci associated with response to SARS-CoV-2 mRNA-1273 vaccine in a cohort of healthy healthcare workers.

Introduction: The COVID-19 pandemic had significant global public health consequences, affecting over 200 countries and regions by 2020. The development and efficacy of specific vaccines, such as the mRNA-1273 (Spikevax) vaccine developed by Moderna Inc., have substantially reduced the impact of the pandemic and mitigated its consequences.

Gut dysbiosis is linked to severe steatosis and enhances its diagnostic performance in MASLD.

Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the leading cause of chronic liver disease globally, with rising prevalence linked to metabolic syndrome (MetS). Excessive liver fat accumulation (steatosis) worsens disease progression and MASLD prognosis. Moreover, gut microbiota dysbiosis might promote steatosis, accelerating the disease progression to severe stages.

The illusory perception of occluded space as empty depends on the occluded area.

Some occluders evoke the compelling impression that the space behind them is empty. Stage magicians use this illusion of absence to produce objects out of thin air. The generic view principle predicts that the illusion of absence should increase with decreasing occluder size.

Circulating microRNAs as biomarkers for ischemic heart disease: a systematic review and gene set enrichment analysis.

Introduction: Ischemic heart disease (IHD) remains a major global health burden, highlighting the urgent need for early, non-invasive diagnostic biomarkers. MicroRNAs (miRNAs), small non-coding RNA molecules that regulate gene expression, have emerged as promising candidates due to their stability in circulation and involvement in cardiovascular processes. This systematic review aimed to evaluate the potential of specific miRNAs as early diagnostic biomarkers in IHD.

Gender Identity and Gender-Related Medical Outcomes of Autistic Young Adults Who Experienced Transgender Identity as Adolescents.

Purpose: We examined gender identity and gender-affirming care outcomes of autistic transgender adolescents followed into young adulthood, as well as relationships between gender-related medical care receipt and mental health across time.

Methods: This longitudinal two-timepoint study was conducted between 2018 and 2024, with 4 years between timepoints. Twenty-seven autistic transgender youth participated, with one lost to follow-up.

Reawakening of a dormant diaphragmatic hernia: a case of delayed post-traumatic presentation.

Traumatic diaphragmatic hernia (TDH) is a rare condition, especially on the right side, often lacking specific symptoms and sometimes manifesting years after the initial trauma. This case report highlights the importance of thorough history-taking, physical examination, and radiological imaging in the diagnosis and treatment of TDH. A 41-year-old male presented with recurrent epigastric pain and respiratory problems, initially treated for gastritis without relief.

Light does not phase shift the circadian clock of subcutaneous adipose tissue in vitro.

The retinal photopigment melanopsin is also expressed in subcutaneous white adipose tissue (scWAT). Through melanopsin, light can modulate scWAT metabolism, but its impact on circadian phase is unclear. In vitro exposure of murine scWAT to bright light at different times over 24 h did not elicit phase shifts, unlike the response to corticosterone.

Innate immune response to AAV-based gene therapy vectors: Mechanisms of complement activation and cytokine release.

Recombinant adeno-associated viruses (rAAV) have emerged as a preferred strategy for gene delivery. However, the immune response to rAAV presents a major limitation, leading to serious adverse events in clinical trials. This study investigates the interaction between rAAV and the innate immune system.

Navigating a Decade of Integrated Care Research in the International Journal of Integrated Care: How Far Have We Come?

Introduction: The scope of integrated care has evolved and broadened the past decades from specialist pathways to incorporate a more holistic approach. To identify such trends in evidence and knowledge, we analysed published papers in the International Journal of Integrated Care over a 10-year period.

Methods: From an initial set of 5.

High- and intermediate-risk pulmonary embolism in pediatric tertiary care centers in the Netherlands.

Background: Pulmonary embolism (PE) response teams are increasingly used in adult care to improve severe PE management. Understanding the epidemiology, treatment, and outcomes of severe PE in children is crucial to assessing the need for pediatric PE response teams in the Netherlands.

Objectives: To evaluate the incidence, management, and short-term outcomes of severe PE in Dutch pediatric patients.

Collagen-elastin dermal scaffolds enhance tissue regeneration and reduce scarring in preclinical models.

Severe scarring is an inevitable consequence of large full-thickness skin wounds, often leading to long-term complications that affect patients' well-being and necessitate extended medical interventions. While autologous split-thickness skin grafts remain the clinical standard for wound treatment, they frequently result in contractures, excessive scarring, and the need for additional corrective procedures. To address these challenges, bioengineered skin substitutes capable of promoting efficient healing while reducing complications are highly desirable.

Using a Blended Approach to Thematic Analysis: A Case Study on Fatherhood and Imprisonment.

Thematic analysis (TA) has become a prominent qualitative research method for identifying patterns in experiences, responses, narratives, and meanings in relation to a research question. In this paper, we provide researchers with an understanding of different approaches to TA and offer practical guidance on conducting a blended approach to TA, which combines the strengths of different TA approaches. Despite their prevalence in the literature, blended approaches to TA are rarely clearly illustrated in research publications.

Mechanically Tunable Biofabricated Channels Enable Mimicking Arterial Pulsatility and Dynamic Tissue Actuation.

Dynamic alteration of blood vessel geometry is an inherent feature of the circulatory system. However, while the engineering of multiscale, branched, and interconnected blood vessels has been well explored, mimicking the dynamic behavior (e.g.

An Unexpected Case of Somatic Mosaicism of the Dutch p16- Founder Variant in the Gene.

is the primary high-risk predisposition gene for familial cutaneous melanoma. In the Netherlands, most carriers of pathogenic germline variants in harbor a unique, population-specific founder variant, c.225_243del, commonly referred to as p16-.

Going beyond SMILES enumeration for data augmentation in generative drug discovery.

Data augmentation can alleviate the limitations of small molecular datasets for generative deep learning by 'artificially inflating' the number of instances available for training. SMILES enumeration - wherein multiple valid SMILES strings are used to represent the same molecules - has become particularly beneficial to improve the quality of molecule design. Herein, we investigated whether rethinking SMILES augmentation techniques could further enhance the quality of design.

Greater Personal Health Agency Is Associated With Greater Patient Receptiveness to Mindset Exercises.

Among 203 patients presenting for musculoskeletal specialty care between November 2023 and January 2024, we measured the relationship of openness to mindset exercises such as cognitive behavioral therapy (training the mind to default to healthier thoughts and feelings about bodily sensations) with levels of personal health agency accounting for other personal factors. Factors associated with greater openness to mindset exercises in linear regression included greater personal health agency (RC = 0.17), younger age (RC = -0.

Enabling and embedding circularity goals in housing cooperatives.

Shifting towards a circular economy in the built environment is considered an important step toward fostering environmentally sustainable and socially resilient cities. Housing cooperatives, established to provide affordable and democratically governed housing, may offer structural advantages for embedding circularity - but their role in circular transitions remains underexplored. This study investigates how cooperative governance may influence the implementation of circular strategies, including circular design, product-service systems, and shared resource models, across different housing types.