Cannabis product exposures reported to Ramathibodi Poison Center, Thailand, during 2018-2022.

Introduction: Cannabis was a category 5 narcotic in Thailand before legalization for medical use in February 2019. In June 2022, it was removed from the narcotics list.

Objectives: To characterize cannabis cases reported to a poison center in Thailand and to analyze the impact of medical and recreational legalization.

Discovery of diverse anellovirus sequences in Thai human sequencing data.

Unlabelled: Anelloviruses are part of the normal human viral flora. Although their diversity in humans has been investigated in many countries, and despite their initial detection in Thailand in 1999, knowledge of Thai anelloviruses remains very limited. This study analyzed 1,175 whole-genome sequencing data sets from Thai individuals to mine for potential anellovirus sequences.

CRISPR/Cas9-Mediated Knockouts of the ALG3 and GNTI in N. benthamiana and Their Application to Pharmaceutical Production.

N-Glycosylation critically influences the efficacy, safety and pharmacokinetic properties of biopharmaceuticals. Plant expression platforms offer multiple advantages for the production of N-glycosylated proteins, but their use is impeded by the presence of plant-specific N-glycan epitopes, which raise concerns of possible immunogenicity to humans. In this study, N-glycoengineered Nicotiana benthamiana plants that produce more homogeneous N-glycans without plant-specific epitopes were generated using multiplex CRISPR/Cas9 genome editing.

Functional and taxonomic dysbiosis of the supragingival plaque metagenome in Behçet's disease.

Background: Behçet's Disease (BD), a complex autoinflammatory disorder, is increasingly linked to microbial dysbiosis, yet the specific microbial signatures and their functional consequences remain incompletely characterized. Elucidating these alterations is crucial for understanding BD pathogenesis.

Objective: To identify distinct microbial community structures and functional potentials in supragingival plaque microbiomes of BD patients versus healthy controls (HC) using high-resolution shotgun metagenomic sequencing.

The Prevalence and Incidence of Depression among Visually Impaired People: A Systematic Review and Meta-Analysis Protocol.

Background: Disability is widely acknowledged as a major contributor to depression, which is a prevalent mental health condition that profoundly affects individuals, especially those with visual impairments.

Objectives: This develops a protocol for a systematic review and meta-analysis aim to examine the prevalence and incidence of depression among visually impaired people globally.

Methods: This systematic review and meta-analysis will be conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA-P) guidelines.

A relapse of acute group B streptococcus meningitis in a healthy adult: Case report and review of the literatures.

(GBS) is a rare cause of meningitis in healthy adults. We report the case of a healthy 33-year-old man with acute GBS meningitis who experienced relapsed high-grade fever and increased intracranial pressure following completing intravenous antibiotics. A short course of corticosteroids, along with additional antibiotics, improved the cerebrospinal fluid (CSF) profile, and no further complications occurred after the recurrent episodes.

Early insulin degludec with continuous intravenous insulin infusion in the management of diabetic ketoacidosis: A randomized controlled trial.

Aims: To determine the effectiveness and safety of early combination therapy with insulin degludec and intravenous insulin infusion (IVII) compared with IVII alone in diabetic ketoacidosis (DKA) management.

Materials And Methods: This prospective, open-label, randomised controlled trial included 80 adults (≥18 years) with DKA. Participants were randomised to either the intervention group, which received early subcutaneous (SC) insulin degludec (0.

Fast and accurate diagnosis of G6PD deficiency via NADPH monitoring using sequential injection analysis coupled with electrochemical detection.

A rapid and automated determination of nicotinamide adenine dinucleotide phosphate (NADPH) is proposed and applied to the evaluation of glucose-6-phosphate dehydrogenase (G6PD) deficiency in real samples. To this end, a sequential injection analyzer with electrochemical detection (SIA-ECD) is proposed with 0.1 mol L Tris-HCl (pH 8.

An unexpected dengue outbreak in Taiwan, 2023: A retrospective analysis of potential risk factors.

Background: Taiwan experienced a major dengue outbreak in 2023 following the relaxation of COVID-19 border controls. The contributing factors remained unclear. This study investigated potential virological, immunological, and clinical drivers.

Enhanced recovery after surgery protocols in gastrectomy with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy for gastric cancer.

Gastric cancer with peritoneal carcinomatosis (PC) remains a formidable challenge in oncological care, especially regarding surgical intervention. Integrating enhanced recovery after surgery (ERAS) protocols into gastrectomy with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy has emerged as a promising approach. This minireview explores the influence of ERAS on surgical and oncological outcomes in this multifaceted procedure.

Correction: Designing adjuvant formulations to promote immunogenicity and protective efficacy of immunoglobulin-like protein a subunit vaccine.

[This corrects the article DOI: 10.3389/fcimb.2022.

Cost-effectiveness of transient elastography for liver fibrosis screening in Thai patients with diabetes mellitus.

Background: The association between diabetes mellitus (DM) and metabolic dysfunction-associated steatotic liver disease (MASLD) is well documented, with DM increasing the risk of developing MASLD and liver fibrosis.

Aim: To evaluate the cost-effectiveness and budget impact of transient elastography (TE) for detecting significant fibrosis in Thai patients with DM.

Methods: We conducted a lifetime cost-utility analysis from a societal perspective, combining a decision tree with a Markov model.

FIGO position statement on the effectiveness and safety of the HPV vaccine.

Cervical cancer, which is primarily caused by persistent infection with oncogenic high-risk human papillomavirus (hrHPV) types, remains a substantial health problem with the highest incidence and mortality rates in low- and middle-income countries (LMICs). HPV vaccination induces a robust immune response against the HPV types included in the vaccine and protects against subsequent development of cervical cancer precursors. Furthermore, countries with high vaccination coverage have observed a significant reduction in the incidence of cervical cancer.

Impact of puberty timing, status and oestradiol on psychotic experiences in the context of exposomic and genomic vulnerability to schizophrenia in female adolescents: longitudinal ABCD study.

Background: During puberty, sex-specific processes shape distinct mental health outcomes. However, research on puberty and psychosis has been limited, and the findings are conflicting.

Aims: To explore how puberty status and timing and oestradiol levels influence psychotic experiences and whether they interact with genetic and exposomic vulnerabilities to schizophrenia in female adolescents.

Evaluation of the Psychometric Properties of the Thai Version of the Cardiac Rehabilitation Barriers Scale.

Objective: To increase participation in cardiac rehabilitation among outpatients with heart disease in Thailand. Factors contributing to low participation are poorly understood. A scale is needed to identify barriers to participation in cardiac rehabilitation.

Mental health and substance use among international migrant workers in the Association of Southeast Asian Nations (ASEAN) countries: a systematic review and meta-analysis.

Previous literature showed that mental health conditions and substance use are prevalent in international migrant workers due to acculturation stress. Given the rapid increase in labour migration within the Association of Southeast Asian Nations (ASEAN) countries, this study aimed to conduct a meta-analysis of the pooled prevalence of mental health conditions and substance use among international migrant workers in ASEAN countries and to identify associated factors. We searched MEDLINE, PubMed, Scopus, and ASEAN Citation Index (ACI) for articles published in English between January 2010 and October 2023.

Plaque and Salivary Fluoride Levels in Preschoolers Following Applications of Silver Diamine Fluoride, Sodium Fluoride Varnish, and Their Combination: A Randomized Clinical Trial.

Background: The amount of fluoride maintained in the oral cavity aids in the remineralization process.

Hypothesis/aim: To evaluate and compare plaque and salivary fluoride levels following applications of silver diamine fluoride (SDF), sodium fluoride varnish (NaFV), or both.

Design: Sixty preschoolers randomly received 38% SDF, 5% NaFV, or both (SDF + NaFV).

Vitamin B levels in older adults with pre-frailty and frailty: the impact of MTHFR and TCN2 polymorphisms and their association with global DNA methylation and physical performance.

Background: Frailty syndrome poses significant challenges in older populations. Understanding the genetic and biochemical factors associated with frailty is essential for effective management strategies.

Methods: In this study, Thai older adults (≥ 60 years, n = 170) were assessed for physical parameters, levels of B vitamins, creatinine, and homocysteine.

Strengthening country ownership of national antimalarial policymaking: insights from Laos, Pakistan and the Solomon Islands.

Background: The concept of "ownership" in global health policymaking broadly assumes that external donors and advisors expect recipient countries to actively steward their national programmes when assistance is provided. This study specifically explored country ownership of national policy decision-making for Plasmodium vivax malaria using three case studies: Laos, Pakistan and the Solomon Islands.

Methods: Yin's comparative case study model, Kingdon's Multiple Streams policy theory guided this analysis.

A prospective comparison of fungal load in paired sputum and bronchoalveolar lavage fluid for Pneumocystis pneumonia diagnosis: Clinical thresholds and diagnostic accuracy.

Background: To compare diagnostic performances and accuracy of non-invasive specimens and bronchoalveolar lavage fluid (BALF) for diagnosing Pneumocystis pneumonia (PCP) in immunocompromised pneumonia patients.

Methods: A prospective study of 112 immunocompromised patients to evaluate P. jirovecii fungal loads in paired sputum and BALF from the same patients.

Early Results of Hypofractionated Chemoradiation in Cervical Cancer with 44 Gy/ 20 F vs 45 Gy/ 25 F: A Phase II, Open-Label, Randomised Controlled Trial (HYPOCx-iRex Trial).

Aims: To compare the safety and efficacy of hypofractionated chemoradiation (HYPO) regimen with a conventional fractionation (CVRT) for locally advanced cervical cancer (LACC).

Materials And Methods: A single-centre, open-label, randomised controlled trial enrolled patients with LACC to receive either HYPO (44 Gy/20 fractions) or CVRT (45 Gy/25 fractions) with intensity-modulated radiotherapy, image-guided adaptive brachytherapy, and concurrent weekly cisplatin. The primary outcome was the incidence of acute and late gastrointestinal (GI) and genitourinary (GU) toxicity assessed using the Common Terminology Criteria for Adverse Events version 5.

Discordance in Drug-Drug Interaction Alerts for Antidotes: Comparative Analysis of Electronic Databases and Interpretive Insights from AI Tools.

Background: Drug-drug interactions (DDIs) are a critical clinical concern, especially when administering multiple medications, including antidotes. Despite their lifesaving potential, antidotes may interact harmfully with other drugs. However, few studies have specifically investigated DDIs involving antidotes.

Enhanced physical, mechanical and barrier properties of chitosan films tannic acid cross-linking.

Growing environmental concerns over the extensive use of petroleum-based polymer packaging have spurred interest in the development of bio-based alternatives. In this work, the incorporation of tannic acid as a cross-linker into chitosan at concentrations of 0-60 wt% was explored. The resulting cross-linking between chitosan chains induced by tannic acid through hydrogen and Schiff-base covalent bonding was confirmed by X-ray photoelectron spectroscopy and gel content measurements.

Immunogenicity and safety of 'Comvigen', a bivalent SARS-CoV-2 vaccine, in comparison to Comirnaty bivalent vaccine in Thailand: a phase 2, non-inferiority randomised trial.

Background: Strengthening mRNA vaccine development in LMICs is essential for enhancing global pandemic preparedness. This study evaluated the safety and immunogenicity of Comvigen, a bivalent SARS-CoV-2 vaccine, in comparison to the Comirnaty bivalent vaccine (Comirnaty).

Methods: This phase II, randomised, open-label, non-inferiority trial was conducted in Thailand across four centres.