A novel ionic liquid 3-(2-hydrazinyl-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride as a derivatization reagent for HPLC-HRMS determination of steroid hormones in urine.

This work demonstrates for the first time the possibility of using a novel ionic liquid 3-(2-hydrazinyl-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride ([HOMI]Cl) as a derivatization reagent for steroid hormones and their subsequent analysis using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry in human urine. Derivatization conditions, such as pH, reagent concentration, reaction time and temperature were evaluated and optimized. A comparative study of [HOMI]Cl and hydroxylamine effectiveness revealed a formation of both syn- and anti-forms of the steroid derivatives for the novel [HOMI]Cl reagent similarly to a derivatization with hydroxylamine.

One-Step Labeling and Purification: Accelerating FAHFA Detection in Vegetable Oils via Chemoselective Probe-Assisted LC-MS.

Fatty acid esters of hydroxy fatty acids (FAHFAs) are emerging bioactive lipids in edible oils, but their trace-level presence and matrix complexity hinder accurate analysis. Here, we designed a novel chemoselective probe piperazine-modified silica () that specifically captures FAHFAs while removing almost totally triglycerides in a one-step labeling reaction. The probe enabled single-batch sample pretreatment within 30 min, integrating capture, purification, and signal amplification.

Effect of Deuterium Concentration in the Incubation Medium on Intracellular Ca Level, Mitochondrial Membrane Potential, and Neuronal Death during Glucose Deprivation.

We studied the effect of increasing and decreasing the deuterium level on the mitochondrial membrane potential (MMP), Ca homeostasis, and neuronal death in the rat cerebellar neurons in vitro. It was found that decreasing deuterium concentration to 100 and 50 ppm was accompanied by gradual depression of MMP and a decrease in the level of cytosolic Ca compared to the baseline values at deuterium concentration 150 ppm. Increasing deuterium concentration to 300 and 750 ppm was associated with an increase in MMP and Ca levels.

Genesis of additional open state zones in the extended polyQ tract of the ATXN2 gene depends on its length and interruptions localization.

The ATXN2 gene is located on chromosome 12q24.1 and encodes the ataxin-2 protein, which is involved in the regulation of RNA metabolism, protein synthesis, and intracellular signaling. The polyQ tract encoding glutamine can expand, which leads to the development of various neurodegenerative diseases.

Cross-cultural data on romantic love and mate preferences from 117,293 participants across 175 countries.

Psychological studies on close relationships have often overlooked cultural diversity, dynamic processes, and potentially universal principles that shape intimate partnerships. To address the limited generalizability of previous research and advance our understanding of romantic love experiences, mate preferences, and physical attractiveness, we conducted a large-scale cross-cultural survey study on these topics. A total of 404 researchers collected data in 45 languages from April to August 2021, involving 117,293 participants from 175 countries.

Genesis of similar collective states in DNA molecules under various viscosity of the medium and external torque.

The nucleotide sequence determines the structural properties of B-DNA; this fact is considered key in recognizing protein binding sites and changing its activity. Our study has established that, using an angular mathematical model, it is possible to determine areas of similar collective states and bends in the nucleotide sequence that arise even under small torque influences. Calculations were performed for two genes that differ slightly in length ( and fragment of the gene to compare).

Interaction Between Glucagon-like Peptide 1 and Its Analogs with Amyloid-β Peptide Affects Its Fibrillation and Cytotoxicity.

Clinical data as well as animal and cell studies indicate that certain antidiabetic drugs, including glucagon-like peptide 1 receptor agonists (GLP-1RAs), exert therapeutic effects in Alzheimer's disease (AD) by modulating amyloid-β peptide (Aβ) metabolism. Meanwhile, the direct interactions between GLP-1RAs and Aβ and their functional consequences remain unexplored. In this study, the interactions between monomeric Aβ40/Aβ42 of GLP-1(7-37) and its several analogs (semaglutide (Sema), liraglutide (Lira), exenatide (Exen)) were studied using biolayer interferometry and surface plasmon resonance spectroscopy.

New latest Famennian (Late Devonian) cystoporate bryozoans from the Bulongguoer section, northwest Xinjiang, China.

A diverse bryozoan fauna was identified from the upper member of the Hongguleleng Formation (Uppermost Famennian) in western Junggar, China. There are fenestrates, cryptostomates, trepostomates and cystoporates, including two new cystoporate species Cheilotrypa gongae sp. nov.

A preliminary study on the role of personal history of infectious and parasitic diseases on self-reported health across countries.

Objectives: Infectious diseases are often associated with decline in quality of life. The aim of this study is to analyze the relationship between personal history of communicable, i.e.

Features of the Defect Structure of the Compositionally Homogeneous Crystal LiNbO:Er(3.1 wt%) and the Gradient Crystal LiNbO:Er and Their Manifestation in the IR Transmission Spectra in the Region of Stretching Vibrations of Hydrogen Atoms of OH-Groups.

Based on the analysis of the IR transmission spectra in the region of stretching vibrations of hydrogen atoms of OH-groups, it was established that the oxygen-octahedral MeO clusters (Me-Li, Nb, vacant octahedron V, impurity ion) of the structure of the compositionally homogeneous crystal LiNbO:Er(3.1 wt%) and the gradient crystal LiNbO:Er(congruent composition by the main components, Er gradient of 0.55 at%/cm) have a shape close to the regular one.

Localization of Potential Energy in Hydrogen Bonds of the Gene.

It is known that a number of neurodegenerative diseases, also called diseases of waiting, are associated with the expansion of the polyQ tract in the first exon of the gene. In the expanded polyQ tract, the probability of occurrence of non-canonical configurations (hairpins, G-quadruplexes, etc.) is significantly higher than in the normal one.

Accounting for the Structure-Property Relationship of Hollow-Fiber Membranes in Modeling Hemodialyzer Clearance.

The relevance of the hemodialysis procedure is increasing worldwide due to the growing number of patients suffering from chronic kidney disease. Taking into account the structure of dialysis polymer membranes is an important aspect in their development to achieve the required performance of hemodialyzers. We propose a new mathematical model of mass transfer that allows hollow-fiber membrane structural parameters to be taken into account in simulating the clearance () of hemodialyzers in a way that does not require difficult to achieve close approximation to the exact geometry of the membrane porous structure.

Love as a Commitment Device : Evidence from a Cross-Cultural Study across 90 Countries.

Given the ubiquitous nature of love, numerous theories have been proposed to explain its existence. One such theory refers to love as a commitment device, suggesting that romantic love evolved to foster commitment between partners and enhance their reproductive success. In the present study, we investigated this hypothesis using a large-scale sample of 86,310 individual responses collected across 90 countries.

New Approaches to the Creation of Highly Efficient Pd-Ag and Pd-Cu Membranes and Modeling of Their Hydrogen Permeability.

Thin-film membranes of Pd-Ag and Pd-Cu alloys capable of releasing hydrogen in a wide temperature range have been developed. The surface activation of the membranes with a nanostructured coating made it possible to intensify hydrogen transport through Pd-containing membranes at low temperatures. This effect was achieved by accelerating limiting surface processes by increasing the active area of the membrane.

Ratio of AT and GC Pairs in the Zones of Open States Genesis in DNA Molecules.

Background: There is an assumption about the presence of a specific nucleotides sequence in DNA molecule, which contributes to the genesis of open states (OS). In addition, it would be logical to assume that OS zones should form in DNA regions with a large proportion of Adenine-Thymine (AT) pairs, since they contain fewer hydrogen bonds than Guanine- Cytosine (GC) base pairs. However, studies have shown that in areas rich in AT pairs, the probability of open states will not always be higher.

Theoretical Study of the Influence of Electroconvection on the Efficiency of Pulsed Electric Field (PEF) Modes in ED Desalination.

Pulsed electric field (PEF) modes of electrodialysis (ED) are known for their efficiency in mitigating the fouling of ion-exchange membranes. Many authors have also reported the possibility of increasing the mass transfer/desalination rate and reducing energy costs. In the literature, such possibilities were theoretically studied using 1D modeling, which, however, did not consider the effect of electroconvection.

Agentic Self-regulation of Capoeira Athletes of Different Sports Qualifications.

Background: In the present study, an attempt was made to address self-regulation at the agentic (higher) level, which contributes a person's success not only in sports, but also in the organization of their own life. A group of capoeiristas was chosen as the sample.

Objective: To identify the features of agentic self-regulation in athletes engaged in capoeira with different levels of sports qualification.

Features of the defect structure of a lithium-gradient nonlinear optical single crystal LiNbO and their manifestation in the Raman spectra.

LiNbO crystal with a lithium composition gradient of Li/Nb = 0.8 wt%/cm (LiNbO) were obtained. A monotonic change in the edge of the UV absorption edge is observed when scanning the surface of the gradient crystal along the growth direction.

Fluctuations in Medium Viscosity May Affect the Stability of the CAG Tract in the Gene.

Trinucleotide repeats are the cause of many neurodegenerative diseases that are currently incurable. In this regard, the question of the causes of occurrence and methods of prevention or treatment of diseases caused by the expansion of repeats in the CAG tract of the gene remains relevant. Previously, it was shown that the frequency of occurrence of additional OS (open states) zones increases with increasing length of the CAG tract, and the value inverse to the frequency correlates with the age of disease onset.

Quantification of cancer biomarkers in urine using volatilomic approach.

Urine analysis is an attractive approach for non-invasive cancer diagnostics. In this study, a procedure for the determination of volatile organic compounds (VOCs) in human urine (acetone, acetonitrile, dimethylsulfide, dimethyl disulfide, dimethyl trisulfide, hexane, benzene, toluene, 2-butanone, 2-pentanone, pentanal) has been described including sample preparation using preconcentration of analytes in sorbent tubes followed by gas chromatography with mass spectrometry (GC-MS). Fractional factorial design and constrained surfaces design were used to optimize preconcentration of VOCs in sorbent tubes.

Diagnosis of Lung Cancer Through Exhaled Breath: A Comprehensive Study.

Objectives: Exhaled breath analysis is an attractive lung cancer diagnostic tool. However, various factors that are not related to the disease status, comorbidities, and other diseases must be considered to obtain a reliable diagnostic model.

Methods: Exhaled breath samples from 646 individuals including 273 patients with lung cancer (LC), 90 patients with cancer of other localizations (OC), 150 patients with noncancer lung diseases (NLD), and 133 healthy controls (HC) were analyzed using gas chromatography-mass spectrometry (GC-MS).