Diagnosis, management and the burden of symptoms of mastocytosis from the physician's perspective: A nationwide study.

Mastocytosis is categorized into cutaneous mastocytosis (CM), mast cell sarcoma and systemic mastocytosis (SM). Within SM, indolent SM (ISM) is the more frequent subtype. Adult patients with CM but without an extracutaneous biopsy are classified as having mastocytosis in the skin (MIS), a provisional diagnosis.

Skin transient receptor potential channels expression and microvascular reactivity to cooling in primary and secondary Raynaud's phenomenon.

Temperature-sensitive Transient Receptor Potential (TRP) channels contribute to modulating skin vascular tone. Their role in Raynaud's Phenomenon (RP) remains unknown. We aimed to investigate TRPs expression in the skin, along with microvascular reactivity to cooling in patients with primary and secondary RP, compared with healthy subjects.

Complex cervicocephalic artery dissections with wide intimal inlet: determinants and implications for endovascular recanalization.

Background And Purpose: Cervicocephalic artery dissection (CCAD) is a well-recognized cause of ischemic stroke. However, complex forms of CCAD, characterized by a wide intimal inlet without a visible intramural hematoma, pose diagnostic challenges and complicate endovascular access to the true lumen when recanalization is required. We aimed to analyze the clinical presentation, outcomes, and feasibility of endovascular treatment of complex CCAD and to propose a novel morphological classification.

Methods for experimentally increasing circulating acyl-CoA-binding protein (ACBP) levels in mice under chronic restraint stress.

Chronic restraint stress (CRS) is a widely recognized model to study stress-induced anorexia and metabolic dysregulation in mice. Acyl-coenzyme A-binding protein (ACBP) has emerged as a critical player in metabolic regulation, with potential implications for stress-related disorders. This study presents two complementary methodologies to artificially elevate circulating Acyl-CoA-binding protein (ACBP) levels in mice under CRS.

Mouse intestine as a useful model for CFTR electrophysiology function analysis.

Cystic fibrosis (CF) is a genetic disorder primarily known for its severe impact on lung function, but it also significantly affects the digestive system, leading to complications such as intestinal blockages, malabsorption, inflammation, and microbial dysbiosis. The study of CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) effects on intestinal physiology is critical for developing new effective treatments. This work highlights the use of the mouse intestine as a valuable model for analyzing cellular electrophysiology and CFTR function.

Orthotopic model of tongue cancer for the study of head and neck squamous cell carcinoma in mice.

Head and neck squamous cell carcinoma (HNSCC) is among the most prevalent human malignancies globally, with approximately 887,000 new cases diagnosed each year. Currently, the standard treatment for HNSCC involves surgery, followed by radiotherapy, chemotherapy and immunotherapy. However, despite these available treatments, the survival rate of patients with HNSCC remains low.

Swiss Webster mice as a model for excessive alcohol binge drinking consumption.

Binge drinking (BD) is a widespread pattern of excessive alcohol consumption among adolescents and young adults with detrimental consequences for brain development. Animal models are essential for investigating the neurobiological mechanisms underlying BD, but selecting an appropriate model is critical to ensure relevance to human behavior. This study aims to validate a murine model of (BD) using Swiss Webster mice.

A protocol for measuring phenotypical facial disease markers in a mouse model of iatrogenic Cushing's syndrome.

Cushing's syndrome is caused by chronic exposure to excessive levels of glucocorticoids. It is characterized by significant phenotypic alterations including increased visceral adiposity and fat deposits on the cheeks, leading to a characteristic 'moon face' appearance. Although glucocorticoid therapy is widespread, its associated side effects are of significant clinical concern.

Wearable airbag for powered two-wheelers: what is the profile of users involved in road traffic crashes and how does it affect their fatality risk?

Background: Traffic fatalities within Powered Two-Wheelers (PTW) users are mostly due to head and thoracic injuries. This study assesses airbag use prevalence among PTW users involved in Road Traffic Crashes (RTCs) in France and aims to estimate airbag effectiveness in protecting against fatality risk.

Methods: A cross-sectional study was conducted using French national police crash data from 1 January 2019 to 31 December 2022, including a total of 69,350 PTW users.

Effect of ECMO Flow Variations on Pulmonary Capillary Wedge Pressure in Patients With Cardiogenic Shock.

Background: The hemodynamic effects of femoro-femoral venoarterial (VA) extracorporeal membrane oxygenation (ECMO) on pulmonary capillary wedge pressure (PCWP) remain poorly defined. High ECMO flow is believed to increase PCWP and the risk of pulmonary edema; yet, supporting in vivo physiological data are lacking.

Objectives: The purpose of this study was to evaluate the impact of incremental femoro-femoral VA-ECMO flow variations on PCWP, hemodynamic, and echocardiographic parameters in patients with cardiogenic shock during the early phase of VA-ECMO support, after stabilization.

Distal Radial Access for Radial Artery Recanalization: Multicenter Outcomes and Stepwise Strategies to Maximize Patency.

Background: Radial artery occlusion complicates transradial access, limiting future access and precluding bypass grafting and dialysis. Distal radial access offers a promising solution by allowing retrograde recanalization of occluded radial arteries.

Objectives: The authors sought to evaluate multicenter outcomes of radial artery recanalization though distal radial access (DRA), focusing on its safety, efficacy, and reproducibility, while identifying procedural strategies to optimize patency.

Accounting for Misclassification of Binary Outcomes in External Control Arm Studies for Unanchored Indirect Comparisons: Simulations and Applied Example.

Single-arm control trials are increasingly proposed as a potential approach for treatment evaluation. However, the limitations of this design restrict its methodological acceptability. Regulatory agencies have raised concerns about this approach, although it is sometimes required in applications based solely on such studies.

EASY-edit: a toolbox for high-throughput single-step custom genetic editing in bacteria.

Targeted gene editing can be achieved using CRISPR-Cas9-assisted recombineering. However, high-efficiency editing requires careful optimization for each locus to be modified, which can be tedious and time-consuming. In this work, we developed a simple, fast and cheap method: Engineered Assembly of SYnthetic operons for targeted editing (EASY-edit) in Escherichia coli.

Assessment of GFR Estimation Methods in Patients with Obesity.

Purpose: Obesity is an independent risk factor for chronic kidney disease, and accurate estimation of the glomerular filtration rate (GFR) is crucial. However, limited data are available on the performance of the European Kidney Function Consortium (EKFC) equation in individuals with overweight or obesity. We evaluated the performance of the EKFC equation by comparing its estimated GFR (eGFR) to values obtained from the 2021 Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation and machine learning (ML) models, using measured GFR (mGFR, obtained via plasma iohexol clearance) as a reference standard in a cohort of patients with overweight or obesity.

Structure activity relationship exploration of imidazo[1,2-a]pyridine series to reverse isoform selectivity and identify potent SIK1 selective inhibitors.

The salt-inducible kinase (SIK) family encompasses three isoforms, SIK1, SIK2, and SIK3, which are members of the AMP-activated protein kinase (AMPK) family of serine/threonine protein kinases. SIK inhibition has emerged as a potential therapeutic approach across multiple indications, as SIKs regulate a diverse set of physiological processes such as metabolism, bone remodeling, immune response, malignancies, skin pigmentation, and circadian rhythm. Within isoform-specific SIK inhibitors there is a need to understand the distinct role of each protein, and here we describe the first SIK1 selective inhibitors.

Global Burden of Psoriasis from 1990 to 2021 and Potential Factors: A Systematic Analysis.

Psoriasis is a chronic, immune-mediated inflammatory skin disorder affecting approximately 100 million people worldwide. This study aimed to understand the global impact of psoriasis on health and economics over the past three decades. we analyzed trends in psoriasis cases, its effects on people's quality of life, and the associated costs.

[Comparison of live-birth rate after Freeze-All strategy between PCOS and non-PCOS patients].

Objective: Polycystic Ovarian Syndrome (PCOS) is a common pathology and the most frequent cause of infertility linked to dysovulation. These patients have a high ovarian reserve and, as a result, oocyte collection is often excessive. Following medically assisted reproduction techniques, i.

Modelling challenges to unlock the power of phototrophic systems for wastewater valorization.

Phototrophic microorganisms are gaining prominence for their dual role in wastewater treatment and resource recovery, converting wastewater into valuable bioproducts. However, their effective deployment needs robust modelling frameworks capable of predicting performance across complex, real-world scenarios. Despite significant advances, key challenges hinder the development and application of such models.

Supplementary Material for Projected Global Clinical, Humanistic, and Economic Impact of Metabolic Dysfunction-Associated Steatohepatitis (MASH): The Cost of Inaction Based on Data from Nine Countries.

Background And Aim: Although the clinical burden of MASH is well known, its economic burden is less well described. We estimated MASH's economic burden in several regions of the world including the US, Germany, Spain, France, Italy, and United Kingdom (UK), Japan, Saudi Arabia, and Brazil over the next two decades.

Methods: A one-year cycle Markov model projected MASH progression from 2021 to 2040, incorporating 2020 prevalent cases and annual incident cases (2021-2040).

Real-world use of terlipressin in cirrhosis and acute kidney injury: frequent use beyond hepatorenal syndrome.

Background & Aims: Terlipressin is indicated to treat hepatorenal syndrome (HRS)-acute kidney injury (AKI), but is likely used outside this primary indication in clinical practice. We aimed to investigate real-world practice patterns on the use of terlipressin in AKI in cirrhosis.

Methods: International prospective study including patients hospitalized for decompensated cirrhosis.

Recent Insights into the Pathomechanisms of Cataract Development in Diabetic Patients.

Diabetes mellitus (DM), a prevalent metabolic disorder characterized by chronic hyperglycemia, disrupts systemic homeostasis and affects multiple tissues, including the eye lens, where cataract is a common diabetic complication. Crystallin proteins constitute the majority of the proteins in the eye lens. One of their characteristics is a long lifespan, which makes them susceptible to the accumulation of physical and chemical damage, particularly in the context of diabetes.

Blood transcriptomic analysis reveals a distinct molecular subtype of treatment resistant depression compared to non-treatment resistant depression.

Treatment-resistant depression (TRD) is a severe condition characterized by chronic and recurrent depressive symptoms, leading to significant morbidity and a considerable socio-economic impact. Genetic and biological studies suggest that TRD is associated with distinct biological characteristics. In this study, we analysed whole-transcriptome differences in 293 patients with major depressive disorder (MDD) to compare TRD (N = 150) vs non-TRD (N = 143) cases.

Facial cues of sickness reduce trustworthiness judgements, with stronger effects in women.

A behavioral defense against disease involves detecting sickness cues in others and responding adaptively, such as by avoiding social interactions. While studies have shown that humans can discriminate sickness cues above chance in faces after sickness induction, whether this discrimination affects approach-avoidance behaviors remains uncertain. Here, we investigated how facial sickness cues influence judgments of trustworthiness, serving as a proxy measure for social avoidance.

Population pharmacokinetics of intravenous ampicillin in awake and anesthetised dogs.

The aim of this study is to describe a population pharmacokinetic model for intravenous ampicillin-sulbactam in awake and anaesthetized dogs in these two treatment scenarios and to compute PK/PD cut-offs (PK/PD). This was a prospective clinical trial in 20 client-owned dogs, either treated by ampicillin after post-surgical infection, or in the context of surgical antimicrobial prophylaxis. All animals received 20mg/kg of ampicillin by slow iv route.

Genotype-phenotype correlations of COL2A1 and COL11A1 patients.

Purpose: To describe and compare the ophthalmological and extra-ophthalmological features of patients with Stickler syndrome due to pathogenic variants in COL2A1 and COL11A1.

Design: Retrospective cross-sectional study nested in a multicentric cohort study.

Methods: Records of patients with a confirmed molecular diagnosis of Stickler syndrome followed-up in the ophthalmology department at Necker-Enfants Malades and Cochin University hospitals (Paris) between 2016 and 2024 were retrospectively reviewed.