Famotidine-loaded chitosan hybridized fibroin nanoparticles exhibit outstanding efficacy in ameliorating peptic ulcer.

Famotidine (FMD) is an H₂-receptor antagonist with limited oral bioavailability and a short plasma half-life (2.5-4 h). Silk fibroin-chitosan nanoparticles (FBN-CS-NPs) represent a novel nanocarrier approach for treating peptic ulcers, combining biocompatibility, mucoadhesiveness, and pH-sensitive release.

Combined serine protease PRSS22 and CEA mRNA analysis identifies the majority of colon cancer patients that recur within 12 years.

Introduction: Proteases play an important role in tumor progression. The predictive efficacy of proteases PRSS3 and PRSS22 mRNA levels for predicting relapse in surgically treated colon cancer (CC) patients was assessed.

Methods: mRNA expression was quantified in 371 half lymph nodes (LNs) from 121 CC patients, 77 control LNs (13 patients), 66 primary colon tumors, and 30 normal colon tissues of these patients.

Discovery of Furopyrimidine-Pyrazole Hybrid Compounds Targeting p53-MDM2 Interaction as Anticancer Agents.

Inhibiting the p53-MDM2 interaction restores the function of the tumour suppressor protein, p53, and offers a promising avenue for anticancer therapies. Herein, a novel series of pyrazoline-derived compounds was developed and synthesised to serve as potential inhibitors of the p53-MDM2 interaction. Scaffold hopping was adopted via replacing the cis-imidazoline core of Nutlin-2 with a pyrazoline core, and molecular docking confirmed the binding orientation of the designed compounds at the p53-MDM2 interaction site.

Harnessing nanotechnology for enhanced wound healing: integrating polymeric nanoparticles with rosuvastatin for targeted therapy.

Purpose: This study investigates the development and evaluation of rosuvastatin-loaded polymeric nanoparticles as a targeted wound-healing therapy. The work aims to overcome solubility and bioavailability limitations and to enhance therapeutic outcomes in cutaneous wound repair.

Methods: Six nanoparticle formulations were prepared via the nanoprecipitation method, varying polymer type and excipient content.

Ancient and dominant: a novel feline immunodeficiency virus subtype "X-EGY" identified in Egyptian cats associated with high prevalence.

Background: Data on the epidemiology and molecular characterization of feline immunodeficiency virus (FIV) in Egypt are limited. This study aimed to estimate FIV prevalence in 240 Egyptian cats during 2022–2024 using three diagnostic techniques: two point-of-care antibody detection kits (Anigen and SNAP) and one end-point PCR targeting the gene. FIV infection is defined as positivity in at least two of the three diagnostic methods or PCR alone confirmed by sequencing, Additionally, FIV-associated clinicopathological abnormalities were assessed, and, for the first time in Egypt, circulating FIV subtypes were identified through partial sequencing and phylogenetic analysis of all gene-positive samples ( = 10), along with 4 additional gene-positive samples.

A Natural Polyphenol, Chlorogenic Acid, Attenuates Obesity-Related Metabolic Disorders in Male Rats via miR-146a-IRAK1-TRAF6 and NRF2-Mediated Antioxidant Pathways.

Chronic high-fat diet (HFD) feeding in male rats causes significant metabolic as well as inflammatory disturbances, including obesity, insulin resistance, dyslipidemia, liver and kidney dysfunction, oxidative stress, and hypothalamic dysregulation. This study assessed the therapeutic effects of chlorogenic acid (CGA), a natural polyphenol, administered at 10 mg and 100 mg/kg/day for the last 4 weeks of a 12-week HFD protocol. Both CGA doses reduced body weight gain, abdominal circumference, and visceral fat accumulation, with the higher dose showing greater efficacy.

The Impact of a Community Pharmacy-Led Deworming-Related Counselling Service: An Interventional Study in a Low-to-Middle Income Country.

Since the current increase in antimicrobial resistance globally, parasitic infectious diseases have become a greater public health crisis than ever before and an absolute priority. The Egyptian community pharmacist, as a health care provider and advisor, has a potential role to play in deworming. : To evaluate the outcomes of community pharmacist-led deworming-related counseling services.

High-Integrity Nanoformulation of Resiquimod (R848) for Dual Autophagy Activation and PD-L1 Modulation in Triple-Negative Breast Cancer.

Triple-negative breast cancer (TNBC) remains a formidable clinical challenge due to its molecular heterogeneity and resistance to conventional therapies. This study presents a high-integrity nanoemulsion (NE) formulation designed to enhance the delivery and stability of the Toll-like receptor 7/8 (TLR7/8) agonist resiquimod (R848) for immunotherapy. Neutral and negatively charged NEs were developed with and without the reactive lipophilic compound ricinoleic acid.

Paclitaxel-induced adverse effects: insights into multi-organ toxicities and molecular mechanisms.

Paclitaxel (PTX), a member of the taxane family of anticancer drugs, is considered one of the most effective chemotherapeutic agents used worldwide. It is widely used in the treatment of solid tumors, including non-small-cell lung, colon, breast, ovarian, prostate, and bladder cancers. Its anticancer activity involves multiple mechanisms: mitotic arrest, mitochondrial damage, and apoptosis induction.

Optimization of Lavender Essential Oil and Phenolic Yield: Advances in Extraction, Metabolomics, and Functional Benefits.

Introduction: Lavender (Lavandula angustifolia) is valued in the food and pharmaceutical industries for its unique aroma, owing to oxygenated monoterpenoids such as linalool, linalyl acetate, lavandulyl acetate, 1,8-cineole, and terpinene-4-ol, which contribute to the quality of lavender essential oil (LEO). It is rich in flavonoids and phenolics like rosmarinic acid. LEO and phenolic yields depend on agronomic attributes, processing techniques, and extraction methods.

Correction: Formulation of zein nanoparticles for augmenting the anti-inflammatory activity of dexketoprofen.

[This corrects the article DOI: 10.3389/fphar.2025.

Hepatoprotective Potential of Green Synthesized Nanoparticles from Peel Extract against HFS Diet-Induced NASH in Mice, Integrated with Chemical Profiling and Molecular Modeling.

is known for its wide variety of secondary metabolites, including essential oil, alkaloids, flavonoids, and phenolic acids, which are considered the reason for its diverse potential medical uses. Herein, extract was prepared using different formulas of metal oxide nanoparticles as ZnO NPs and MgO NPs with 22.5 ± 1.

Targeting miRNAs in renal cell carcinoma: emerging therapeutic strategies.

About one-third of renal cell carcinoma (RCC) patients present with metastatic disease upon diagnosis because of the retroperitoneal location of the kidneys, which causes many tumors to stay asymptomatic. Besides, shortly after 5 years following successful curative surgery, nearly 30% of individuals develop distant cancer metastases and recurrences. This is mostly attributable to the complex and diverse characteristics of the tumor microenvironment.

Effect of Kaempferol against Biofilm Formation by Klebsiella pneumoniae Clinical Isolates.

Background: Biofilm production is a key factor in the development of antibiotic resistance in multidrug-resistant Klebsiella pneumoniae (K. pneumoniae), a significant contributor to healthcare-associated infections (HAIs). Kaempferol, a flavonoid, is widely recognized for its ability to combat various microorganisms.

Evaluation of postextraction wound healing of immediate complete dentures using a modified inflammation proliferation remodeling scale following platelet-rich fibrin and xenograft application: A randomized clinical trial.

Statement Of Problem: Immediate denture insertion after dental extraction results in problems with socket healing, negatively affecting patient responses. Platelet-rich fibrin (PRF) and xenografts have been introduced to overcome such healing problems, but their clinical outcomes require further study.

Purpose: The purpose of this randomized clinical study was to assess postextraction healing and correlate it with bone volume changes during immediate denture insertion after socket treatment using PRF and xenografts.

Metagenomic insight into drought-induced changes in the Egyptian wheat rhizosphere microbiome.

Wheat is one of the most important cereal crops and an important source of food for billions of people worldwide. However, drought stress can pose a real threat to its productivity and lead to significant yield losses, especially in Egypt. The rhizospheric microbiome of wheat can play an important role in drought stress and help wheat to respond to this abiotic stress.

CD4 Discordance as a Predictor of Liver Fibrosis in People Coinfected with Human Immune Deficiency Virus/Hepatitis C Virus: A Cross-Sectional Study.

Liver fibrosis presents a unique diagnostic challenge in people coinfected with hepatitis C virus (HCV) and human immune deficiency virus (HIV). This study aimed to explore the association between CD4 discordance and liver fibrosis in that population, alongside assessing the predictive power of different models for significant fibrosis. A cross-sectional study was conducted on 198 adult people with HIV/HCV coinfection.

The possible role of lycopene on the cerebellum of albino rat prenatally exposed to valproic acid: Animal model of autism spectrum disorder.

Autism is a serious neurodevelopmental disorder with a rising global prevalence. Prenatal exposure to valproic acid (VPA), a common antiepileptic drug, is associated with autism in offspring. Lycopene, a potent antioxidant and anti-inflammatory compound, may counteract VPA-induced neurotoxicity.

Synthesis, characterization, antimicrobial and antibiofilm potential of green copper silicate and zinc silicate nanoparticles: kinetic study and reaction mechanism determination.

The study explored an eco-friendly method to synthesize Cu silicate and zinc silicate nanoparticles (NPs) and characterize them using analytical instruments like transmission electron microscopy, DLS analysis, Zeta potential, EDX elemental analysis, and scanning electron microscopy with mapping. We tested how well Cu silicate and Zn silicate NPs can fight bacteria that cause wound infections and unicellular pathogenic fungi by checking their antimicrobial properties, the smallest amount needed to stop growth (minimum inhibitory concentration), and their ability to prevent biofilm formation. To investigate a potential mechanism of antimicrobial behavior, we applied the membrane leakage experiment.

Development of a novel and eco-friendly Alginate@ZIF-8/Moringa Oleifera nano-biocomposite (Alg@ZIF-8/MO-NBC) for environmental remediation through efficient methylene blue removal from aqueous solutions.

The persistent presence of synthetic dyes such as methylene blue (MB) in industrial wastewater poses serious environmental and health hazards due to their toxicity, stability, and resistance to conventional treatment methods. In this study, we report the development of a novel and eco-friendly Alginate@ZIF-8/Moringa Oleifera nano-biocomposite (Alg@ZIF-8/MO-NBC) for the effective adsorption of MB from aqueous solutions. The composite was fabricated by incorporating Zeolitic Imidazolate Framework-8 (ZIF-8) into a matrix of sodium alginate and carbonized Moringa Oleifera seed powder.

A multichannel biosensor integrated with a controller board for standard addition voltammetric signal analysis of cardiac troponin-I.

Conventional miniaturized immunosensors featuring a single working electrode are often time-consuming due to the lengthy immunoassay procedures they require. Additionally, they necessitate background correction of the voltammetric signal. To overcome these challenges, a multichannel electrochemical immunosensor with six working electrodes has been developed.

Formulation and Evaluation of Astaxanthin-Loaded Invasomes as Therapeutic Approaches for Alzheimer's Disease Induced in Rats: Role of SIRT-1/BDNF/miRNA-134/GSK-3β Signaling.

Alzheimer's disease (AD) is a progressive age-dependent neurodegenerative disorder associated with oxidative brain damage, disrupted neuronal transmission, memory loss, and behavioral changes, with aluminum being a key environmental risk factor that exacerbates its effect. The aim of this study is to enhance the therapeutic potential of astaxanthin (AST) in Alzheimer's disease by formulating it into invasomal carriers, with special emphasis on SIRT-1/BDNF/miRNA-134/GSK-3β signaling in an AD-like rat model caused by aluminum chloride (AlCl) at a dose of 100 mg/kg/day for 60 days. Optimum AST-loaded invasomes (AST-LI) were prepared using a formulation of phospholipid: ethanol: cineole as 300 mg: 0.

Correction: Mohammed et al. Alvespimycin Exhibits Potential Anti-TGF-β Signaling in the Setting of a Proteasome Activator in Rats with Bleomycin-Induced Pulmonary Fibrosis: A Promising Novel Approach. 2023, , 1123.

In the original publication [...

Zebrafish in the spotlight: expanding Frontiers in toxicology and drug discovery.

Zebrafish () has emerged as a valuable model organism in toxicology and drug discovery research. This article provides an overview of the significant contributions of zebrafish to advancing our understanding of toxicology and drug development. Zebrafish offers several advantages, including high fecundity, transparent embryos, and genetic tractability, making it an ideal system for studying drug toxicity and efficacy.