Evaluation of Bio-Stimulation Effects of Various Dermal Fillers: Hyaluronic Acid-Based Polynucleotides and Microparticle Fillers in Mouse Model.

Background: Dermal fillers have become prevalent in aesthetic surgery, offering noninvasive solutions for addressing signs of aging and enhancing facial features.

Methods: In this study, the in vivo administration of dermal fillers, including a hyaluronic-cross-linked filler (HA-R), a PDLLA-HA combined filler (HA-PDLLA), a microparticle PDO filler (PDO), a PN filler extracted from salmon milt (PN), and pure hyaluronic acid (HA), along with PBS as a control, was evaluated over a 12 week period.

Results: HA-R exhibited sustained volume retention, contrasting with rapid volume loss observed with PN, and gradual dissolution of PDO by week 12. Histological evaluation revealed varying inflammatory responses, with PDO showing notable alleviation of inflammation after 8 weeks. MT staining demonstrated increased collagen formation post-injection, peaking at 4 weeks for most fillers. Immunofluorescent staining highlighted the initial promotion of collagen synthesis by PDO and HA-PDLLA, followed by a subsequent decline after 8 weeks.

Conclusion: This study provided comprehensive insights into the biophysical properties and efficacy of various dermal fillers in stimulating collagen production. PDO and HA-PDLLA initially demonstrated efficacy in promoting collagen synthesis, but limitations were noted in sustaining long-term collagen production. Understanding the dynamics of filler administration and its impact on tissue response is essential for optimizing outcomes in aesthetic surgery practice.

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