Observed reduction in false measles IgM positivity using an ELISA kit containing RF absorbent.

J Microbiol Methods

Department of Medical Microbiology Laboratory, Istanbul Provincial Health Directorate Public Health Services Department, No. 1 Public Health Laboratory, Istanbul, Türkiye. Electronic address:

Published: August 2025


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Article Abstract

This study evaluated whether an Rheumatoid Factor RF-absorbent ELISA assay reduces false-positive measles IgM results in low-prevalence settings. Among 76 initially reactive samples, only 7 remained positive or borderline upon retesting, demonstrating a 91 % reduction. These findings support the use of RF-blocking diagnostics to improve test specificity and surveillance accuracy.

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http://dx.doi.org/10.1016/j.mimet.2025.107238DOI Listing

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