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This study evaluated whether an Rheumatoid Factor RF-absorbent ELISA assay reduces false-positive measles IgM results in low-prevalence settings. Among 76 initially reactive samples, only 7 remained positive or borderline upon retesting, demonstrating a 91 % reduction. These findings support the use of RF-blocking diagnostics to improve test specificity and surveillance accuracy.
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http://dx.doi.org/10.1016/j.mimet.2025.107238 | DOI Listing |
Hum Vaccin Immunother
December 2025
Department of Planned Immunization, Chaoyang District Centre for Disease Control and Prevention, Beijing, China.
Mumps is a vaccine-preventable disease. Following Beijing's implementation of a three-dose MMR vaccination strategy, incidence rates declined significantly. However, the current 21-d quarantine period lacks evidence-based support, resulting in low compliance and conflicts between healthcare providers and patients.
View Article and Find Full Text PDFJ Microbiol Methods
August 2025
Department of Medical Microbiology Laboratory, Istanbul Provincial Health Directorate Public Health Services Department, No. 1 Public Health Laboratory, Istanbul, Türkiye. Electronic address:
This study evaluated whether an Rheumatoid Factor RF-absorbent ELISA assay reduces false-positive measles IgM results in low-prevalence settings. Among 76 initially reactive samples, only 7 remained positive or borderline upon retesting, demonstrating a 91 % reduction. These findings support the use of RF-blocking diagnostics to improve test specificity and surveillance accuracy.
View Article and Find Full Text PDFJAMA Netw Open
August 2025
Mayo Clinic Vaccine Research Group, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota.
Importance: India's goal of measles elimination remains unmet, as evidenced by significant recent outbreaks.
Objective: To identify seroprevalence rates among vaccinated children and to examine demographic factors that influence antibody responses.
Design, Setting, And Participants: This cross-sectional study included a highly vaccinated cohort of Indian children from the states of Kerala and Tamil Nadu between 2018 and 2023.
Health Sci Rep
September 2025
Laboratoire National de Santé Publique Brazzaville Republic of the Congo.
Background And Aims: The only previous sequence report of measles virus from the Republic of the Congo (RoC) dates back to samples collected in 2000. To update the molecular epidemiology of measles virus in the RoC, our study genotyped measles virus strains circulating in the country from January 2023 to May 2024.
Methods: A total of 584 serum or plasma samples were collected by the National Laboratory of Public Health through its nationwide measles surveillance activities.
Virol J
August 2025
National Institute for Infectious Diseases "Lazzaro Spallanzani" -IRCCS, Laboratory of Virology, Rome, Italy.
Purpose: In 2023-2024, measles cases progressively increased in Europe. Multiple outbreaks were reported and viral strains with three-nucleotide mutations potentially compromising diagnostic testing were identified. We analyzed the first cases of measles reported in Lazio (Central Italy) from September 2023 to March 2024, combining molecular characterization and phylogenetic analysis with epidemiological investigation to identify transmission chains and evaluate the sensitivity of PCR tests adopted on circulating viral strains.
View Article and Find Full Text PDF