Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Cholesterol is an essential component of the mammalian cell membrane. Elucidating the dynamics of intracellular cholesterol is extremely important for understanding the mechanisms underlying life phenomena and diseases. The fluorescent cholesterol analog, R-Chol, has a fluorescent group at the 3-OH position of free cholesterol, which is less toxic and helpful for studying free cholesterol trafficking in live cells. In the present study, we determined the details of R-Chol dynamics in live cells. R-Chol was taken up by the plasma membrane within 10 min and then incorporated into the cells by endocytosis. Free cholesterol in late endosomes/lysosomes is transported to the endoplasmic reticulum via NPC1 proteins. In Npc1-deficient CHO cells, R-Chol accumulates in late endosomes/lysosomes. R-Chol accumulation in Npc1-deficient cells was attenuated by the overexpression of Rab9, which regulates intracellular transport from late endosomes to the Golgi apparatus. These results indicate that R-Chol incorporated via endocytosis is transported to late endosomes/lysosomes and then to the Golgi apparatus and endoplasmic reticulum by NPC1-dependent and NPC1-independent vesicular trafficking.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.abb.2025.110600 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association of cardiovascular risks and carotid stiffening at ultrafast ultrasound imaging in young populations.
Eur Radiol
September 2025
Department of Ultrasound, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, China.
Objectives: To evaluate the predictive role of carotid stiffening, quantified using ultrafast pulse wave velocity (ufPWV), for assessing cardiovascular risk in young populations with no or elevated cardiovascular risk factors (CVRFs).
Materials And Methods: This study enrolled 180 young, apparently healthy individuals who underwent ufPWV measurements. They were classified into three groups: the CVRF-free group (n = 60), comprising current non-smokers with untreated blood pressure < 140/90 mmHg, fasting blood glucose (FBG) < 7.
Single-Cell Membrane Molecular Cartography Enabled by Nanoengineered VUV-LDI Mass Spectrometry Imaging.
Anal Chem
September 2025
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Deciphering the multicomponent of cell membranes at the single-cell level is critical for understanding pathological mechanisms such as tumor metastasis, yet remains technically daunting due to the membrane's nanoscale thickness and ultralow molecular abundance. Here, we introduce a surface-assisted vacuum ultraviolet laser desorption-ionization mass spectrometry imaging (SAVUVDI-MSI) platform that overcomes long-standing challenges of cytoplasmic interference and insufficient sensitivity. Leveraging the nanoscale depth profiling capability of VUV-LDI, we achieve precise ablation of a single-cell membrane.
View Article and Find Full Text PDFMeibum - a lipid-rich secretion produced by holocrine Meibomian glands (MG) - plays a central role in maintaining ocular surface homeostasis. Previously, changes in MG lipidomes induced by inactivation of critical genes of meibogenesis, such as and others, were shown to cause MG-dysfunction- and dry eye-like signs in mice. Here, we describe the impact of the lipid composition of meibum on its physiological properties, specifically thermotropic/melting characteristics, using various wild type and mutant animals, and compare them with meibum of healthy humans and patients with abnormal meibum.
View Article and Find Full Text PDFCorrelation between thyroid sensitivity indices and bone metabolism in newly diagnosed middle aged and elderly patients with type 2 diabetes mellitus with normal thyroid function.
BMC Endocr Disord
September 2025
Department of Endocrinology and Metabolism, Jiangxi Medical College, The Second Affiliated Hospital, Nanchang University, Nanchang City, 330006, Jiangxi Province, China.
Objective: To investigate the association between thyroid hormone sensitivity indices and bone metabolism markers in newly diagnosed middle-aged and elderly type 2 diabetes mellitus (T2DM) patients with normal thyroid function.
Method: We retrospectively analyzed 350 newly diagnosed T2DM patients (≥ 45 years), stratified by bone mineral density into Group A (normal bone density group) and Group B (low bone mass and osteoporosis group). General data and clinical biochemical parameters were collected: free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), 25-hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), osteocalcin (OC), bone-specific alkaline phosphatase (BALP), serum calcium (Ca), serum phosphorus (P), fasting plasma glucose (FPG), glycosylated hemoglobinA1c (HbA1c), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum creatinine (SCr), serum uric acid (SUA), and estimated Glomerular Filtration Rate (eGFR).
Prevention of Atopic Dermatitis in High-Risk Infants: A Review of the Role of Lipid-Based Barrier Repair Therapy.
Pediatr Dermatol
September 2025
James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, Ohio, USA.
Background/objective: Growing evidence highlights the role of physiological lipids, namely ceramides, cholesterol, and free fatty acids, in maintaining skin barrier function and preventing atopic dermatitis (AD). Current evidence on the efficacy, safety, and clinical relevance of stratum corneum (SC) lipid-based therapies to prevent AD and increase skin barrier integrity in high-risk infants was reviewed and synthesized.
Methods: Searches with key words lipid-based therapy, atopic dermatitis, infant, and prevention were conducted to identify papers using PubMed, Embase, Cochrane Library, and Scopus databases from January 2000 to June 2024.