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Article Abstract
Simultaneous acquisition of fluorescent voltage and intracellular calcium signals requires spectrally distinct indicators and a high-speed imaging setup. Here, we present a protocol for the optical mapping of these key physiological parameters in human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) monolayers. We describe steps for using spectrally compatible voltage and calcium sensors involving fluorescent dyes and genetically encoded sensors. We also detail procedures for building a driver capable of controlling light-emitting diodes (LEDs) in synchrony with a high-speed camera during image acquisition.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397942 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2025.104044 | DOI Listing |
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