Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
, a major foodborne pathogen, causes a serious infection. Therefore, better detection methods are needed to prevent outbreaks. Herein, a colorimetric/fluorescent dual-mode aptasensor using (GlcN), AuNPs, and QD- was developed for its rapid detection. The probe was an -specific aptamer with cDNA attached to carboxyl magnetic beads (Apt-cDNA-CMBs). In the presence of , the aptamer binds to it, making cDNA detach and adsorb onto AuNPs, inhibiting (GlcN)-induced aggregation and keeping the AuNPs solution red. Meanwhile, dispersed AuNPs quench QDs, leading to reduced fluorescence. Without L. , AuNPs aggregate, turning the solution blue, and QD fluorescence remains unchanged. The lowest detection limits (LODs) were 4.21 CFU/mL (colorimetry) and 8.89 CFU/mL (fluorescence). The aptasensor showed excellent recoveries in beef (85.11%-107.53%) and salmon (86.21%-109.98%). This dual-mode aptasensor offered a promising solution for simple, rapid, and reliable on-site detection of
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12362018 | PMC |
| http://dx.doi.org/10.1016/j.isci.2025.113203 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A colorimetric fluorometric dual-mode aptasensor for aflatoxin B detection driven by Au@CeO core-shell nanozymes.
Mikrochim Acta
September 2025
Henan Agricultural University, Zhengzhou, 450002, China.
A dual-mode aptasensor was engineered for aflatoxin B (AFB) detection by functional integration of peroxidase-mimetic Au@CeO core-shell nanostructures with emissive carbon dots (CDs). The Au@CeO nanocomposite, synthesized via spontaneous redox reaction, exhibited enhanced peroxidase-like activity due to abundant Ce/oxygen vacancies facilitating hydroxyl radical generation. The aptasensor utilizes a competitive binding mechanism, where AFB competed with immobilized Au@CeO-CDs-Apt1 probes for binding sites, resulting in inversely proportional colorimetric and fluorescent signals.
View Article and Find Full Text PDFHigh-Affinity Aptamer Selection for Microbial Transglutaminase and Application in Colorimetric-SERS Dual-Mode Detection.
Anal Chem
September 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
Microbial transglutaminase (MTGase) from is widely used in food processing to improve texture but poses health risks due to immune reactions and masking raw material quality. This study developed a sensitive detection method using a high-affinity aptamer (S1) selected via Capture-SELEX with a dissociation constant of 24.32 ± 5.
View Article and Find Full Text PDFIntegrated Photoelectrochemical-Fluorescence Dual-Mode Aptasensor for Sensitive Detection of Fumonisin B1.
Anal Chem
September 2025
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
Long-term intake of fumonisin B1 (FB1) can pose a threat to human health. It is essential to develop an accurate and sensitive method to detect FB1. In this work, a photoelectrochemical-fluorescence (PEC-FL) dual-mode aptamer sensing platform was proposed for FB1 analysis.
View Article and Find Full Text PDFAn electrochemical/colorimetric sensor for 5-methyltetrahydrofolate based on a screened specific aptamer and DNA walker-driven CRISPR/Cas12a cascade amplification.
Biosens Bioelectron
December 2025
Wuxi Maternity and Child Health Care Hospital, Women's Hospital of Jiangnan University, Jiangnan University, Wuxi, 214002, China. Electronic address:
5-Methyltetrahydrofolate (5-MTHF), the primary bioactive form of folate (vitamin B9), played a vital role in human metabolism. In this work, an electrochemical/colorimetric dual-mode aptasensor for 5-MTHF was constructed by combining a DNA Walker-driven CRISPR-Cas12a trans-cleavage system. A 5-MTHF aptamer D1a was obtained through Capture-SELEX with subsequent trimming of non-binding regions, which exhibiting high affinity and specificity.
View Article and Find Full Text PDFA colorimetric/fluorescent dual-mode aptasensor for the rapid and sensitive detection of .
iScience
September 2025
Department of Food and Biological Sciences, Agricultural College, Yanbian University, Yanji 133002, China.
, a major foodborne pathogen, causes a serious infection. Therefore, better detection methods are needed to prevent outbreaks. Herein, a colorimetric/fluorescent dual-mode aptasensor using (GlcN), AuNPs, and QD- was developed for its rapid detection.
View Article and Find Full Text PDF