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Blood flow visualization can improve intraoperative surgical decision-making. A dual-display laparoscopic imaging system (DLIS) integrating laser speckle contrast imaging (LSCI) simultaneously provides color RGB and tissue blood flow imaging in real-time. Traditional LSCI uses a fixed-size sliding window for contrast calculation, while adaptive methods mitigate noise for better blood flow visualization. In this paper, we utilize the G channel of RGB images, which reveals fine vascular structures, to generate guide images distinguishing vascular and non-vascular regions. Adaptive pixel selection criteria are applied based on specific region types. In vivo experiments demonstrate that our method improves signal-to-noise ratio in global and boundary area compared to conventional approaches, and captures microvascular perfusion with detail comparable to that in the G channel.
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http://dx.doi.org/10.1364/OE.557618 | DOI Listing |
CNS Neurosci Ther
September 2025
Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Aims: Sustained neuroinflammation following ischemic stroke impedes post-injury tissue repairment and neurological functional recovery. Developing innovative therapeutic strategies that simultaneously suppress detrimental inflammatory cascades and facilitate neurorestorative processes is critical for improving long-term rehabilitation outcomes.
Methods: We employed a microglia depletion-repopulation paradigm by administering PLX5622 for 7 days post-ischemia; followed by a 7-day withdrawal period to allow microglia repopulation.
Magn Reson Med
September 2025
Department of Biomedical Engineering, University of California, Davis, Davis, California, USA.
Purpose: This study sought to determine the intrasession repeatability of the diffusion-weighted (DW) arterial spin labeling (ASL) sequence at different postlabel delays (PLDs).
Methods: We first performed numerical simulations to study the accuracy of the two-compartment water exchange rate (Kw) fitting model with added Gaussian noise for DW PLDs at 1500, 1800, and 2100 ms. Ten young, healthy participants then underwent a structural T scan and two intrasession in vivo DW ASL scans at each PLD on a 3T MRI.
Abnormal immune responses are common clinical features in septic patients. γδ T cells, as innate immune cells, play an important role in host defense, immune surveillance and homeostasis. However, the immune characteristics of γδ T cells in pediatric sepsis remains remain poorly understood.
View Article and Find Full Text PDFBackground: Space exploration has progressed significantly, with increased human presence in orbit, the development of space stations, and the planning of increasingly prolonged missions. However, the space environment poses substantial physiological challenges, particularly for the cardiovascular system. According to NASA's Human Research Program, the five primary risks associated with human spaceflight are: (1) microgravity, (2) ionizing cosmic radiation, (3) isolation and confinement, (4) closed environmental systems, and (5) the great distance from Earth.
View Article and Find Full Text PDFMagn Reson Med
September 2025
Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand.
Purpose: Tracer kinetic models are used in arterial spin labeling (ASL); however, deciding which model parameters to fix or fit is not always trivial. The identifiability of the resultant system of equations is useful to consider, since it will likely impact parameter uncertainty. Here, we analyze the identifiability of two-compartment models used in multi-echo (ME) blood-brain-barrier (BBB)-ASL and evaluate the reliability of the fitted water-transfer rate ).
View Article and Find Full Text PDF