Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Accurate detection of single-nucleotide variations (SNVs) plays a pivotal role in medical diagnosis. Herein, by integrating Cas12a and Cas13a to simultaneously detect wild-type and mutated sites on a single RNA strand, we developed an innovative one-pot SNV analysis method, namely iCasdrop, which is capable of reducing non-specific signals induced by the wild type sequence and achieving ratiometric calibration of SNVs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d5cc03931b | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Granzyme B activated near-infrared-II ratiometric fluorescent nanoprobe for early detection of tumor response to immunotherapy.
Nat Commun
August 2025
The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, P. R. China.
Non-invasive optical imaging tools for early detecting anti-tumor immune responses are crucial for precision cancer immunotherapy. However, current probes often suffer from low imaging depth, single imaging channel, and inadequate quantification, hindering their in vivo applications. Here we develop a rare-earth-based NIR-II fluorescence ratiometric nanoprobe (DCGA) for in vivo real-time, precise, and non-invasive visualization of granzyme B (GzmB) activity, a key effector in T cell-mediated antitumor immunity, for early prediction of immunotherapy efficacy.
View Article and Find Full Text PDFSelf-Enhanced Ratiometric ECL Strategy Based on a Dual-Emission Ce/Tb-MOF Luminophore with Spatially Resolved Coreactant Delivery for Ultrasensitive Pro-GRP Detection.
Anal Chem
September 2025
School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Dual-signal-output ratiometric electrochemiluminescence (ECL) platforms offer a promising solution to address the instability and inaccuracies associated with single-signal ECL detection. However, most existing ratiometric systems still rely on two independent luminophores, inevitably increasing the system complexity and compromising the detection accuracy. In this study, a bimetallic dual-ligand ECL luminophore, Ce/Tb-MOF, was synthesized via a solvothermal approach, exhibiting distinct anodic and cathodic ECL emissions from a single luminophore.
View Article and Find Full Text PDFCharge-Rearrangement Averaged Dye Design for Precise, Real-Time, and Portable Monitoring of Complex Solvent Environments.
Angew Chem Int Ed Engl
August 2025
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438, China.
Although solvatochromic materials provide visual function to differentiate solvents, they fail to precisely monitor complex solvent environments where solvent composition varies dynamically, because of an insensitive absorptional shift in these environments. Relatively, their fluorescence signal change, which should be more sensitive, has always been inefficient, resulting from inherent challenges in excited-state dynamics. Here, we present a charge-rearrangement averaged dye design, instead of traditional donor-acceptor structures, to enable a high locally excited or twisted intramolecular charge transfer emission efficiency.
View Article and Find Full Text PDFCyanine-Based Dual-Emission Sensor for Ratiometric NAD(P)H Imaging in Cells, Renal Tissues, and Entire Organisms.
ACS Appl Bio Mater
August 2025
Department of Chemistry, Michigan Technological University, Houghton, Michigan 49931, United States.
Monitoring the dynamic fluctuations of NADH and NADPH, key coenzymes in intracellular redox homeostasis and bioenergetics, is essential for understanding both normal physiology and disease pathology. However, precise, real-time imaging of NAD(P)H levels in complex biological systems remains a major challenge due to interference from endogenous fluorescence and the limitations of intensity-based sensors. We present a newly engineered dual-emission fluorescent sensor, sensor , which overcomes these obstacles by offering ratiometric detection with high specificity and sensitivity.
View Article and Find Full Text PDFSmartphone-Powered Pdot Biosensors for Quantitative Phenylketonuria Screening at the Point of Care.
Adv Healthc Mater
August 2025
Department of Biomedical Engineering, Xiangya School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
Current clinical management of hyperphenylalaninemia and phenylketonuria (PKU) lacks accessible, real-time diagnostic tools. To address this gap, a portable biosensing system integrating semiconducting polymer dot (Pdot) technology with miniaturized optics for rapid phenylalanine quantification is developed. The biosensor leverages phenylalanine dehydrogenase to enzymatically convert phenylalanine into NADH, enabling ratiometric fluorescence detection through electron transfer-mediated quenching of Pdots by NADH.
View Article and Find Full Text PDF