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A user-friendly, portable, low-cost readout system for the on-site or point-of-care characterization of chemo- and biosensors based on an electrolyte-insulator-semiconductor capacitor (EISCAP) has been developed using a thumb-drive-sized commercial impedance analyzer. The system is controlled by a custom Python script and allows to characterize EISCAP sensors with different methods (impedance spectra, capacitance-voltage, and constant-capacitance modes), which are selected in a user interface. The performance of the portable readout system was evaluated by pH measurements and the detection of the antibiotic penicillin, hereby using EISCAPs consisting of Al/p-Si/SiO/TaO structures and compared to the results obtained with a stationary commercial impedance analyzer. Both the portable and the commercial systems provide very similar results with almost perfectly overlapping recorded EISCAP signals. The new portable system can accelerate the transition of EISCAP sensors from research laboratories to commercial end-user devices.
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http://dx.doi.org/10.3390/s25092681 | DOI Listing |
Adv Sci (Weinh)
September 2025
Department of Orthodontics, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, NHC Key Laboratory of Digital Stomatology, NMPA Key
Clear aligners offer aesthetic and comfort advantages in orthodontics, yet their ability to deliver effective forces relies heavily on empirical judgment or large-scale optical scanning, lacking real-time quantitative evaluation. Integrating pressure sensors into aligners is a promising solution, but challenges in miniaturization, multi-dimensional sensing, measurement accuracy, and biocompatibility hinder clinical application. Here, an all-in-one Orthodontic Force Acquisition System (OFAS) is presented that enables real-time, 3D force monitoring using a cross-shaped iontronic sensing array and an origami-inspired, wireless battery-free readout circuit miniaturized for single-tooth placement.
View Article and Find Full Text PDFBiosens Bioelectron
September 2025
Department of Biological Sciences, Faculty of Science, National University of Singapore, 117543, Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore; Precision Medicine Translational Research Programme (TRP), Yong Loo Lin School
DNAzymes possessing kinase-like activities have long held theoretical promise, yet their practical implementation has remained significantly limited. Notably, DNAzyme kinase 1 (DK1), discovered over two decades ago, exhibits a unique self-phosphorylation capability upon encountering specific substrates like ATP, but its broad-based and programmable applications have not yet been fully realized. In this study, we innovatively couple DK1's autophosphorylation mechanism with the PfAgo to establish a novel programmable cascade sensing platform named RASTEN (Robust pfAgo-based Strategy for POC Testing Non-nucleic Acid and Nucleic Acid).
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address:
Aptamers are single-stranded DNA or RNA oligonucleotides that can bind to specific target molecules with high affinity and specificity. Fluorescence DNA aptamer-based biosensors (aptasensors) have emerged as powerful analytical tools for detecting diverse targets, ranging from food contaminants to disease biomarkers, owing to their exceptional specificity, high sensitivity, and cost-effectiveness. This review systematically summarizes recent advances in the design strategies of fluorescence aptasensors, focusing on three key approaches: (1) fluorescence resonance energy transfer-based signal amplification, (2) nanomaterial-enhanced probes, and (3) multi-channel platforms for simultaneous detection.
View Article and Find Full Text PDFData generated in studies of cellular regulatory systems are often qualitative. For example, measurements of signaling readouts in the presence and absence of mutations may reveal a rank ordering of responses across conditions but not the precise extents of mutation-induced differences. Qualitative data are often ignored by mathematical modelers or are considered in an ad hoc manner, as in the study of Kocieniewski and Lipniacki (2013) [Phys Biol 10: 035006], which was focused on the roles of MEK isoforms in ERK activation.
View Article and Find Full Text PDFBiology (Basel)
July 2025
Neurobiology and Molecular Medicine Unit, IRCCS Fondazione Stella Maris, Calambrone, 56128 Pisa, Italy.
Early diagnosis is critical for the effective management of neurodegenerative disorders, and retinal alterations have emerged as promising early biomarkers due to the retina's close developmental and functional link to the brain. The zebrafish (Danio rerio), with its rapid development, transparent embryos, and evolutionarily conserved visual system, represents a powerful and versatile model for studying retinal degeneration. This review discusses a range of behavioral assays-including visual adaptation, motion detection, and color discrimination-that are employed to evaluate retinal function in zebrafish.
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