Publications by authors named "Kawtar Ettayri"
Recent advances in DNA aptamer-based fluorescence biosensors from design strategies to diverse applications and future challenges: A review.
Int J Biol Macromol
September 2025
Aptamers are single-stranded DNA or RNA oligonucleotides that can bind to specific target molecules with high affinity and specificity. Fluorescence DNA aptamer-based biosensors (aptasensors) have emerged as powerful analytical tools for detecting diverse targets, ranging from food contaminants to disease biomarkers, owing to their exceptional specificity, high sensitivity, and cost-effectiveness. This review systematically summarizes recent advances in the design strategies of fluorescence aptasensors, focusing on three key approaches: (1) fluorescence resonance energy transfer-based signal amplification, (2) nanomaterial-enhanced probes, and (3) multi-channel platforms for simultaneous detection.
View Article and Find Full Text PDFOxytetracycline (OTC), a broad class of antibacterial medication, has been widely utilized in poultry husbandry and fish farming. However, the presence of OTC residues in the food has become a matter of great concern. These residues have the potential to induce the emergence and spread of bacterial resistance, which can have far-reaching consequences for public health.
View Article and Find Full Text PDFArticle Synopsis
- Researchers developed a highly sensitive fluorescence aptasensor using 2D nanomaterials like MoS that minimizes background signals for detecting aflatoxin B1 (AFB1).
- The aptasensor utilizes a new dicationic fluorophore, VLM, which strengthens fluorescence when it binds to target molecules, enhancing detection accuracy.
- Results show that the aptasensor can detect AFB1 concentrations from 2-3000 ng/mL with an impressive limit of detection (LOD) of 0.006 ng/mL, and it provides quick results in about 74 minutes, making it a practical tool for rapid contamination testing.
Article Synopsis
- Researchers have developed dual-color DNA-templated silver nanoclusters that act as a fluorescent probe without the need for labels, incorporating a specific G5-linker.
- This innovation allows for the flexible design of aptasensors that utilize aptamers with a C5-linker, which can be tailored for different detection purposes.
- The technology enables multiplexed detection, meaning multiple targets can be identified simultaneously, enhancing its application versatility.
Article Synopsis
- This study examined thiol-capped CdZnTe quantum dots (QDs) as effective fluorescence signal tags for aptasensing, using various ligands to enhance their properties.
- The research found that NAC-capped CdZnTe QDs offered the best performance in terms of stability and interference resistance, particularly when incorporated into dendritic mesoporous silica nanoparticles (DMSNs).
- An advanced aptasensor for detecting aflatoxin B1 was developed, achieving impressive sensitivity and the ability to customize for different targets by changing the aptamer sequence.
Background: The importance of multi-target simultaneous detection lies in its ability to significantly boost detection efficiency, making it invaluable for rapid and cost-effective testing. Photoelectrochemical (PEC) sensors have emerged as promising candidates for detecting harmful substances and biomarkers, attributable to their unparalleled sensitivity, minimal background signal, cost-effectiveness, equipment simplicity, and outstanding repeatability. However, designing an effective multi-target detection strategy remains a challenging task in the PEC sensing field.
View Article and Find Full Text PDF