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Article Abstract
Tetracycline is extensively used in aquaculture as a therapeutic agent that needs to be monitored due to food safety concerns. Aptasensing has been revealed as a suitable diagnostic platform for tetracycline sensing in food matrix due to its quick, low cost and robust nature. But, the colorimetric aptasensing of tetracycline employing the peroxidase activity of gold nanoparticles (AuNPs) to 3,3,5,5-tetramethylbenzidine (TMB) was unsuitable until now owing to the aptamer-specific alkaline binding buffer. The present study developed a method with an optimized reaction protocol diminishing the inhibitory effect of binding buffer on the sensor probe (AuNPs-aptamer + TMB + HO). The overall peroxidase activity of the sensor probe was only inhibited by tetracycline through selective adsorption on the AuNPs-aptamer complex. The peroxidase inhibition percentage in the test range of 0.01 to 0.5 mg L tetracycline gave a logarithmic response (R, 0.99) with a detection limit of 0.017 mg L which is less than the prescribed limit (0.1 mg L) set by EU and FSSAI. The developed sensing system in fish muscle showed high recovery (111-115 %) with great potential for rapid detection of tetracycline in fish muscle.
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| http://dx.doi.org/10.1016/j.ymeth.2025.04.017 | DOI Listing |
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