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Background: Rice diseases that are not detected in a timely manner may trigger large-scale yield reduction and bring significant economic losses to farmers.
Aims: In order to solve the problems of insufficient rice disease detection accuracy and a model that is lightweight, this study proposes a lightweight rice disease detection method based on the improved YOLOv8. The method incorporates a full-dimensional dynamic convolution (ODConv) module to enhance the feature extraction capability and improve the robustness of the model, while a dynamic non-monotonic focusing mechanism, WIoU (weighted interpolation of sequential evidence for intersection over union), is employed to optimize the bounding box loss function for faster convergence and improved detection performance. In addition, the use of a high-resolution detector head improves the small target detection capability and reduces the network parameters by removing redundant layers.
Results: Experimental results show a 66.6% reduction in parameters and a 61.9% reduction in model size compared to the YOLOv8n baseline. The model outperforms Faster R-CNN, YOLOv5s, YOLOv6n, YOLOv7-tiny, and YOLOv8n by 29.2%, 3.8%, 5.2%, 5.7%, and 5.2%, respectively, in terms of the mean average precision (mAP), which shows a significant improvement in the detection performance.
Conclusion: The YOLOv8-OW model provides a more effective solution, which is suitable for deployment on resource-limited mobile devices, to provide real-time and accurate disease detection support for farmers and further promotes the development of precision agriculture. © 2025 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.8790 | DOI Listing |
Anal Chem
September 2025
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing 314001, China.
Despite the promise of electrochemical biosensors in amplified nucleic acid diagnostics, existing high-sensitivity platforms often rely on a multilayer surface assembly and cascade amplification confined to the electrode interface. These stepwise strategies suffer from inefficient enzyme activity, poor mass transport, and inconsistent probe orientation, which compromise the amplification efficiency, reproducibility, and practical applicability. To address these limitations, we report a programmable dual-phase electrochemical biosensing system that decouples amplification from signal transduction.
View Article and Find Full Text PDFMol Pharm
September 2025
Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Tissue factor (TF) has emerged as a promising target for the diagnosis and treatment of hepatocellular carcinoma (HCC). However, there is limited data available on TF-related PET imaging for longitudinal monitoring of the pathophysiological changes during HCC formation. Herein, we aimed to explore the TF-expression feature and compare a novel TF-targeted PET probe with F-FDG through longitudinal imaging in diethylnitrosamine (DEN)-induced rat HCC.
View Article and Find Full Text PDFAnal Chem
September 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361
Rolling circle amplification (RCA) has revolutionized nucleic acid detection owing to its isothermal simplicity. However, over two decades of clinical application have been hampered by off-target amplification and incompatibility with double-stranded DNA (dsDNA). Herein, a strategy, specifically cleavage of rationally designed DNA/RNA chimeric hairpin preprimer by dsDNA-targeted CRISPR/Cas12a to rlease ssRNA for initiating RCA (SCOPE-RCA), is proposed for nucleic acid identification of African swine fever virus (ASFV).
View Article and Find Full Text PDFRetina
September 2025
Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, Freiburgstrasse 15, CH-3010.
Purpose: To evaluate inter-grader variability in posterior vitreous detachment (PVD) classification in patients with epiretinal membrane (ERM) and macular hole (MH) on spectral-domain optical coherence tomography (SD-OCT) and identify challenges in defining a reliable ground truth for artificial intelligence (AI)-based tools.
Methods: A total of 437 horizontal SD-OCT B-scans were retrospectively selected and independently annotated by six experienced ophthalmologists adopting four categories: 'full PVD', 'partial PVD', 'no PVD', and 'ungradable'. Inter-grader agreement was assessed using pairwise Cohen's kappa scores.
J Infect Dis
September 2025
Biological Sciences, Sunnybrook Research Institute, Sunnybrook Hospital, Toronto, Ontario, Canada.
Background: Tecovirimat (TPOXX) is an antiviral authorized for the treatment of mpox infections in Canada, but recent clinical trials found it has no impact on symptom duration.
Methods: We conducted a prospective cohort study of individuals diagnosed with mpox in Toronto, Canada. Skin lesion swabs were collected weekly to quantify infectious monkeypox virus (MPXV) shedding through cell culture.