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Article Abstract
Expression of double-stranded RNAs (dsRNAs) in plants is an emerging strategy to efficiently control insects. RNA interference (RNAi)-mediated pest control takes advantage of double-stranded RNA that can suppress the expression of one or more insect genes that encode key proteins. Virus-induced gene silencing (VIGS) is a useful tool for plant expression of dsRNAs to control pests without altering the plant's genome. Trehalase (TRE) and chitin synthase (CHS) are very important in insects. In this study, we first demonstrated that spraying dsRNAs targeting and increased the mortality rate of the peach aphid treated with the pathogenic fungus . When dsRNAs targeting and were expressed in plants via VIGS, the expression of and was reduced in aphids, and their fertility and survival rates were decreased. These results indicate that VIGS-mediated RNA interference is a powerful approach to effectively control aphids, and aphids had a higher mortality rate when was sprayed.
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| http://dx.doi.org/10.1021/acs.jafc.4c09681 | DOI Listing |
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