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Article Abstract
In this study, the gene , associated with resistance to ear rot, was identified through transcriptome data analysis of the maize inbred line J1259. The gene was subsequently cloned and its function was investigated. The gene comprises an open reading frame of 525 base pairs, encoding a protein of 175 amino acids. was overexpressed in Arabidopsis and the mutant in maize, and they were subjected to q-PCR and measurements of antioxidant enzyme activities (POD, SOD, CAT, MDA), electrical conductivity, and chlorophyll content. The results indicate that the expression of is up-regulated upon infection by , with significant differences observed in the activities of POD, SOD, CAT, MDA, electrical conductivity, and chlorophyll content. The study found that is localized in the nucleus and interacts with and . Trypan blue staining revealed that the stained area in the mutant of maize was significantly larger than that in B73. The expression of is closely associated with resistance to maize ear rot.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901443 | PMC |
| http://dx.doi.org/10.3390/plants14050737 | DOI Listing |
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