Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A surface-enhanced Raman scattering (SERS) aptasensor was developed using gold nanostars (Au NSs) and FeO@Au nanoparticles (NPs) for the highly sensitive detection of aflatoxin B (AFB). Au NSs were modified by the Raman reporter 4-aminothiophenol (PATP) and then coupled with cDNA to act as the capture probes (Au NSs@PATP-cDNA). FeO@Au NPs were modified with the AFB aptamer (AFB Apt) and used as signal probes (FeO@Au NPs-AFB Apt). The SERS peak of PATP at 1078 cm was used for quantitative analysis. When the core-satellite nanostructures (FeO@Au NPs-AFB Apt/cDNA-Au NSs@PATP) were self-assembled by oligonucleotide hybridization, the SERS intensity was significantly enhanced. When AFB was present, AFB Apt specifically binds to AFB, causing the FeO@Au NPs-AFB Apt and Au NSs@PATP-cDNA to dissociate, resulting in a decrease in the SERS intensity measured after magnetic separation. Under optimal conditions, the limit of detection (LOD) of AFB can be reduced to 0.24 pg/mL. This is attributed to the high affinity of AFB Apt, excellent magnetic separation characteristics, and multiple SERS hotspots. The assay has been validated to perform well in recovery and accuracy by evaluating spiked samples (rice, corn, and wheat) and positive samples (corn, brown rice, and wheat).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00604-025-07040-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A core-satellite self-assembled SERS aptasensor used for ultrasensitive detection of AFB.
Mikrochim Acta
February 2025
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu, China.
A surface-enhanced Raman scattering (SERS) aptasensor was developed using gold nanostars (Au NSs) and FeO@Au nanoparticles (NPs) for the highly sensitive detection of aflatoxin B (AFB). Au NSs were modified by the Raman reporter 4-aminothiophenol (PATP) and then coupled with cDNA to act as the capture probes (Au NSs@PATP-cDNA). FeO@Au NPs were modified with the AFB aptamer (AFB Apt) and used as signal probes (FeO@Au NPs-AFB Apt).
View Article and Find Full Text PDFLateral flow assay for simultaneous detection of multiple mycotoxins using nanozyme to amplify signals.
Food Chem
December 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address:
Article Synopsis
- Co-contamination of multiple mycotoxins poses greater health risks, making simultaneous detection vital.
- A new three-channel aptamer-based lateral flow assay (Apt-LFA) was developed to detect aflatoxin M, aflatoxin B, and ochratoxin A quickly and accurately.
- This method utilizes gold-iridium nanozyme for signal amplification, achieving detection limits of 0.39 ng/mL for AFM, 0.36 ng/mL for AFB, and 0.82 ng/mL for OTA, with high recovery rates from real samples.
A sandwich-type photoelectrochemical biosensor based on Ru(bpy) sensitized InS for aflatoxin B detection.
Analyst
July 2024
Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Ministry of Education), Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials of Anhui Province, Key Laboratory of Functional Inorganic Materials of Anhui Province, School of Chemistry & Chemical E
Aflatoxin B (AFB), classified as a class I carcinogen, is a widespread mycotoxin that poses a serious threat to public health and economic development, and the food safety problems caused by AFB have aroused worldwide concern. The development of accurate and sensitive methods for the detection of AFB is significant for food safety monitoring. In this work, a sandwich-type photoelectrochemical (PEC) biosensor for AFB detection was constructed on the basis of an aptamer-antibody structure.
View Article and Find Full Text PDFFluorescent solid-state strips based on SiO shell-stabilized perovskite nanocrystals applying for magnetic aptasensing detection of aflatoxin B toxin in food.
Food Chem
August 2024
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China; Key Laboratory of Food Quality and Health of Tianjin, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address:
In this work, the perovskite fluorescent nanocrystals (CsPbBr) were successfully synthesized and wrapped with SiO shell, utilized for the assembly of solid-state detection strip capable of conveniently and specifically detection of aflatoxin B (AFB). The SiO coating aimed to enhance the stability of CsPbBr nanocrystals. The resulting CsPbBr@SiO material exhibited remarkable fluorescence properties, and further self-assembled onto solid-state plate, generating AFB-specific quenched fluorescence at a specific wavelength of 515 nm.
View Article and Find Full Text PDFA facile dual-mode SERS/fluorescence aptasensor for AFB detection based on gold nanoparticles and magnetic nanoparticles.
Spectrochim Acta A Mol Biomol Spectrosc
July 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food
Aflatoxin B (AFB) is a virulent metabolite secreted by Aspergillus fungi, impacting crop quality and posing health risks to human. Herein, a dual-mode Raman/fluorescence aptasensor was constructed to detect AFB. The aptasensor was assembled by gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), while the surface-enhanced Raman scattering (SERS) and fluorescence resonance energy transfer (FRET) effects were both realized.
View Article and Find Full Text PDF