Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Mechanical stretch-mediated tissue expansion is effective for obtaining extra skin and soft tissue required for the repair of defects or reconstruction of surface organs. Understanding the cellular and molecular mechanisms and identifying hub genes and key cell types associated with skin expansion could help predict the success of skin growth during expansion procedures.
Methods: We analyzed murine chip sequencing data and single-cell sequencing data available from the Gene Expression Omnibus database. Based on the differentially expressed and epithelial-mesenchymal transition-related genes, random forest and protein-protein interaction network analysis identified hub genes for predicting skin regeneration in tissue expansion. The fate of the cell subpopulations, expression of hub genes in different cell types, and their communication were also assessed.
Results: Five genes, integrin beta 5 (), tropomyosin 1 (), secreted frizzled-related protein-1 (), , and insulin-like growth factor binding protein 2 (), were identified as having the greatest impact on prediction accuracy. These hub genes were primarily enriched in the Notch and phosphoinositide 3-kinase-AKT pathways. Immune cell infiltration analysis further revealed that mast cell infiltration was significantly higher in the expanded skin group than that in the control group. According to single-cell data, the interactions between epithelial cells, stem cells, and other cell types were higher in the expanded skin group than those in the control group. Moreover, , and were highly expressed in all epithelial and stem cell subgroups.
Conclusions: The hub genes, , and their associated signaling pathways such as Notch and Wnt signaling and functions in key cell subsets highlight prospective therapeutic strategies to enhance skin growth under mechanical expansion. Moreover, mast cell activation and infiltration may trigger immune responses in the expanded skin, which requires further investigation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655345 | PMC |
| http://dx.doi.org/10.3389/fimmu.2024.1306353 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comprehensive Analysis of Differentially Expressed Genes and Immune Infiltration in Burn Injury: Key Biomarkers and Pathways.
J Burn Care Res
September 2025
Department of Burn Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Background: Burn injuries trigger complex immune responses and gene expression changes, impacting wound healing and systemic inflammation. Understanding these changes is crucial for identifying biomarkers and therapeutic targets.
Methods: We analyzed two GEO datasets (wound tissue (GSE8056) and blood (GSE37069)) to identify differentially expressed genes (DEGs) in burn injury samples versus controls.
Uncovering Overlapping Gene Networks and Potential Therapeutic Targets in Osteoporosis and COVID-19 Through Bioinformatics Analysis.
Int J Endocrinol
August 2025
Department of Geriatrics, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen 361000, Fujian, China.
Osteoporosis is a progressive bone disease characterized by reduced bone density and deterioration of bone microarchitecture, predominantly affecting the elderly population. The ongoing COVID-19 pandemic has introduced additional challenges in osteoporosis management, potentially due to systemic inflammation and direct viral impacts on bone metabolism. This study aims to identify common differentially expressed genes (DEGs) and key molecular pathways shared between osteoporosis and COVID-19, with the goal of uncovering potential therapeutic targets through bioinformatics analysis.
View Article and Find Full Text PDFExploring ischemic stroke based on the ferroptosis perspective: ECH1 may serve as a new biomarker and therapeutic target.
Front Neurosci
August 2025
School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.
Background: Ischemic stroke (IS), the leading stroke subtype (∼87%), arises from vascular occlusions, triggering brain necrosis through ischemia-reperfusion injury. Ferroptosis, an iron-driven cell death via Fe-mediated lipid peroxidation, is implicated in IS pathology. This study demonstrates that enoyl-coA hydrolase 1 (ECH1) may serve as a peripheral biomarker and therapeutic target for IS based on ferroptosis signaling.
View Article and Find Full Text PDFIdentification of gene signatures and potential pharmaceutical candidates linked to COVID-19-related depression based on gene expression profiles.
Front Pharmacol
August 2025
School of Health Management, Zhejiang Pharmaceutical University, Ningbo, China.
Background: Acute and long-term mental health disorders correlate with coronavirus disease 2019 (COVID-19). The underlying mechanisms responsible for the coexistence of COVID-19 and depression remain unclear, and more research is needed to find hub genes and effective therapies. The main objective of this study was to evaluate gene-expression profiles and, identify key genes, and discovery potential therapeutic agents for co-occurrence in COVID-19 and major depressive disorder (MDD).
View Article and Find Full Text PDFSuppresses Colorectal Cancer Progression by Inhibiting Epithelial-Mesenchymal Transition via Regulation.
Oncol Res
September 2025
Department of General Surgery, Shanghai Pudong New Area People's Hospital, Shanghai, 201299, China.
Background: Colorectal cancer (CRC) is common and deadly, often leading to metastasis, challenging treatment, and poor outcomes. Understanding its molecular basis is crucial for developing effective therapies.
Aims: This study aimed to investigate the role of Myosin Heavy Chain 11 (MYH11) in CRC progression, especially its effects on epithelial-mesenchymal transition (EMT) and cell behavior, and to explore its potential regulation by the EMT transcription factor zinc finger E-box binding homeobox 1 (ZEB1).