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A gamma cell unit is used for gamma irradiation of small volume samples. Due to the sample volume, there is a dose distribution, which is imperative to be known for appropriate irradiation. In this study, samples of agarose Fricke gel dosimeters were prepared for the dose mapping of the gamma cell unit available in our laboratory. To place the dosimeters at precise locations within the irradiation chamber of the gamma cell unit, a specific phantom was designed and fabricated. In this way, the dose distribution was estimated using optical absorbance measurement of the gel dosimeters. For verification, isodose curves were also obtained using Monte Carlo simulations. The results indicate that the central east-west and north-south points in the irradiation chamber have the highest and lowest dose values, respectively. The experimental and simulation ratios of the dose uniformity was calculated to be 1.53 and 1.48, respectively. The accuracy of the dosimeter performance and the system's calibration quality are confirmed by the good agreement between the simulation and experimental results.
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http://dx.doi.org/10.1016/j.apradiso.2024.111631 | DOI Listing |
Cancer Immunol Immunother
September 2025
Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, China.
Objective: CircRNAs are involved in cancer progression. However, their role in immune escape in non-small cell lung cancer (NSCLC) remains poorly understood.
Methods: This study employed RIP-seq for the targeted enrichment of circRNAs, followed by Western blotting and RT-qPCR to confirm their expression.
Nature
September 2025
Department of Neurology, Brigham and Women's Hospital, Boston, MA, USA.
Neural activity is increasingly recognized as a crucial regulator of cancer growth. In the brain, neuronal activity robustly influences glioma growth through paracrine mechanisms and by electrochemical integration of malignant cells into neural circuitry via neuron-to-glioma synapses. Outside of the central nervous system, innervation of tumours such as prostate, head and neck, breast, pancreatic, and gastrointestinal cancers by peripheral nerves similarly regulates cancer progression.
View Article and Find Full Text PDFJ Immunother Cancer
September 2025
Harold C Simmons Comprehensive Cancer Center, The University of Texas Southwestern Medical Center, Dallas, Texas, USA
Background: While highly efficacious for numerous cancers, immune checkpoint inhibitors (ICIs) can cause unpredictable and potentially severe immune-related adverse events (irAEs), underscoring the need to understand irAE biology.
Methods: We used a multidimensional approach incorporating single-cell RNA sequencing, mass cytometry, multiplex cytokine assay, and antinuclear antibody (ANA) profiling to characterize the peripheral immune landscape of patients receiving ICI therapy according to irAE development.
Results: Analysis of 162 patients revealed that individuals who developed clinically significant irAEs exhibited a baseline proinflammatory, autoimmune-like state characterized by a significantly higher abundance of CD57 T and natural killer (NK) T cells, plasmablasts, proliferating and activated CXCR3 lymphocytes, CD8 effector and terminal effector memory T cells, along with reduced NK cells and elevated plasma ANA levels.
J Immunother Cancer
September 2025
Division of Hematology & Oncology, Department of Medicine, School of Medicine, University of California, Irvine, California, USA
Background: γδ T cells possess unique immunological features including tissue tropism, major histocompatibility complex-independent antigen recognition, and hybrid T/natural killer cell properties that make them promising candidates for cancer immunotherapy. However, the therapeutic potential of Vδ1 γδ T cells, particularly when engineered with chimeric antigen receptors (CARs), remains underexplored in solid tumors such as pancreatic cancer (PC), largely due to their low abundance in peripheral blood and challenges in ex vivo expansion. This study aims to directly compare the preclinical safety and efficacy among CAR-engineered Vδ1 γδ T cells, Vδ2 γδ T cells, and conventional αβ T cells.
View Article and Find Full Text PDFPhysiol Plant
September 2025
Department of Microbiology, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand, India.
Environmental sustainability is seriously threatened by the discharge of wastewater containing hazardous heavy metals (such as Cr, Cd, As, Hg, etc.). The utilization of microalgae has recently come to light as a viable, environmentally acceptable method for removing heavy metals from contaminated sites.
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