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As a crop with significant medicinal value and nutritional components, the market demand for bitter melon continues to grow. The diversity of bitter melon shapes has a direct impact on its market acceptance and consumer preferences, making precise identification of bitter melon germplasm resources crucial for breeding work. To address the limitations of time-consuming and less accurate traditional manual identification methods, there is a need to enhance the automation and intelligence of bitter melon phenotype detection. This study developed a bitter melon phenotype detection model named CSW-YOLO. By incorporating the ConvNeXt V2 module to replace the backbone network of YOLOv8, the model's focus on critical target features is enhanced. Additionally, the SimAM attention mechanism was introduced to compute attention weights for neurons without increasing the parameter count, further enhancing the model's recognition accuracy. Finally, WIoUv3 was introduced as the bounding box loss function to improve the model's convergence speed and positioning capabilities. The model was trained and tested on a bitter melon image dataset, achieving a precision of 94.6%, a recall of 80.6%, a mAP50 of 96.7%, and an F1 score of 87.04%. These results represent improvements of 8.5%, 0.4%, 11.1%, and 4% in precision, recall, mAP50, and F1 score, respectively, over the original YOLOv8 model. Furthermore, the effectiveness of the improvements was validated through heatmap analysis and ablation experiments, demonstrating that the CSW-YOLO model can more accurately focus on target features, reduce false detection rates, and enhance generalization capabilities. Comparative tests with various mainstream deep learning models also proved the superior performance of CSW-YOLO in bitter melon phenotype detection tasks. This research provides an accurate and reliable method for bitter melon phenotype identification and also offers technical support for the visual detection technologies of other agricultural products.
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http://dx.doi.org/10.3390/plants13233329 | DOI Listing |
Microorganisms
August 2025
Food Animal Environmental Systems Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Bowling Green, KY 42101, USA.
We utilized silver nanoparticles synthesized from bitter melon () extracts for testing against the common agricultural pathogen . The synthesized nanoparticles were characterized and confirmed as silver nanoparticles by using ultraviolet spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy analysis. The results show that AgNPs were effective against ATCC25922 strain.
View Article and Find Full Text PDFPlant Physiol Biochem
August 2025
Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. Electronic address:
Powdery mildew (PM) is one of the most serious diseases in balsam pear. MLO (Mildew Resistance Locus O) is a key factor in the response of plants to PM infection, but its regulation mechanism remains poorly understood. In this study, overexpression of McMLO7b (MLO7b in Momordica charantia L) was found to potentially enhance Arabidopsis susceptibility to PM, confirming that McMLO7b acts as a susceptibility factor during PM infection.
View Article and Find Full Text PDF3 Biotech
September 2025
Division of Crop Protection, ICAR - National Research Centre for Banana (NRCB), Tiruchirappalli, India.
Unlabelled: Mosaic disease, caused by whitefly-transmitted begomoviruses, significantly threatens bitter gourd cultivation in India. This study identified and characterized the complete genome of coccinia mosaic Virudhunagar virus (CoMViV) from symptomatic bitter gourd samples collected in Oddanchatram block of Dindigul district, Tamil Nadu, using rolling circle amplification and sequencing. A loop-mediated isothermal amplification (LAMP) protocol was optimized by targeting regions of the CoMViV AV1 and AV2 genes.
View Article and Find Full Text PDFAsian Pac J Cancer Prev
August 2025
Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.
Objective: This study investigates the pharmacokinetic interactions and anticancer potential for rosuvastatin in combination with Momordica charantia (M. charantia) extract through both in vitro and in vivo models.
Methods: A validated high-performance liquid chromatography (HPLC) method (R² = 0.
Nutrients
July 2025
Department III of Functional Sciences, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Plant supplements are frequently used by diabetes mellitus (DM) patients in the management of their disease. The present study aimed to identify the prevalence of plant supplement use in DM patients from Romania and to evaluate patients' practices, profiles, and beliefs regarding plant supplements and the impact of their use on glycemic control. A cross-sectional online survey was conducted among Romanian diabetic patients.
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