Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Dalbavancin, an antimicrobial lipoglycopeptide, is authorized in Europe for treating acute bacterial infections of the skin and skin structures in adults and pediatric patients aged 3 months and older. However, off-label dosing regimens have been proposed for various indications beyond acute bacterial infections of the skin and skin structures. This study presents a novel bioanalytical method using liquid chromatography-tandem mass spectrometry to quantify dalbavancin in low-volume plasma samples (50 μL).
Methods: The method underwent validation in accordance with international guidelines for bioanalytical method validation and was applied to 9 clinical samples obtained from pediatric and young adult patients undergoing dalbavancin therapy. Liquid chromatography-tandem mass spectrometry analyses were conducted at the G. Gaslini Institute in Genoa, Italy, utilizing an Ultimate 3000 ultra high performance liquid chromatography system coupled to a TSQ Quantiva Triple Quadrupole system (Thermo Fisher Scientific, Milan, Italy). The analytical procedure involved the addition of deuterated dalbavancin as internal standard and a rapid extraction from 50 µL of human plasma, followed by chromatographic separation on a Thermo Scientific Accucore Polar Premium column. Accurate quantification of the analyte was achieved through multiple reaction monitoring detection.
Results: The assay exhibited linearity within the concentration range of 0.66-400 mcg/mL in plasma, demonstrating accuracy and reproducibility in the absence of matrix effects. Stability testing was conducted on both quality controls and real samples to establish a robust protocol under real-life conditions.
Conclusions: This fast and reliable dalbavancin quantitation method could improve current pediatric clinical practice by enabling data collection for future dose recommendations in special patient populations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/FTD.0000000000001260 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
HT SpaceM: A high-throughput and reproducible method for small-molecule single-cell metabolomics.
Cell
September 2025
Molecular Systems Biology Unit, European Molecular Biology Laboratory, Heidelberg, Baden-Württemberg 69117, Germany; Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA; Metabolomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Baden-Württe
Single-cell metabolomics (SCM) promises to reveal metabolism in its complexity and heterogeneity, yet current methods struggle with detecting small-molecule metabolites, throughput, and reproducibility. Addressing these gaps, we developed HT SpaceM, a high-throughput SCM method combining cell preparation on custom glass slides, small-molecule matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (MS), and batch processing. We propose a unified framework covering quality control, characterization, structural validation, and differential and functional analyses.
View Article and Find Full Text PDFPopulation pharmacokinetics of a single oral dose of gabapentin identifies rapid plasma clearance in rehabilitated Pacific harbor seal pups (Phoca vitulina richardii).
Am J Vet Res
September 2025
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA.
Objective: Determine the pharmacokinetic parameters of a single dose of gabapentin administered orally in healthy weaned Pacific harbor seal (HS) pups (Phoca vitulina richardii).
Methods: In the spring of 2023, rehabilitated HS pups were enrolled in this pharmacokinetic study. Seals were administered 10 mg/kg of gabapentin orally in a fish.
Exposure Profiles to Bisphenols and Their Impact on Reproductive and Thyroid Hormones in Pregnant Women.
Arch Med Res
September 2025
University of Algiers, Algiers, Algeria.
Background: Bisphenols are emerging pollutants of health concern. Exposure to bisphenols may impact hormone physiology, particularly during pregnancy, when the body is more vulnerable to disruptions.
Objective: This study aimed to identify bisphenol exposure profiles in pregnant women and to explore associations between urinary levels of these compounds and disruptions in reproductive and thyroid hormone levels during pregnancy.
Discrimination of isomeric dihydroflavone and chalcone by the boosting optimal collision energy-liquid chromatography-tandem mass spectrometry: A case study of pharmacokinetics in honey-fried licorice.
J Chromatogr B Analyt Technol Biomed Life Sci
September 2025
Research Center for Chinese Medicine Analysis and Transformation, Beijing University of Chinese Medicine, Beijing 100029, China; Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address:
The occurrence of isomeric dihydroflavone and chalcone, two important subfamilies of flavonoid class, extensively happens in herbal medicines. However, identical MS/MS spectra make the identity confirmation a tough job, the complexity will be further boosted in biological samples. Inspired by that isomers possess distinct inherent physicochemical parameters, optimal collision energy (OCE), which is positively correlated with the bond dissociation energies (BDEs), was evaluated towards differentiating isomeric dihydroflavone and chalcone.
View Article and Find Full Text PDFRunning exercise alleviates depressive-like behaviors through the activation of PINK1-Parkin mediated mitophagy in mice exposed to chronic social defeat stress.
Psychiatry Res
September 2025
Department of Nautical Psychology, Faculty of Psychology, Naval Medical University, Shanghai, PR China. Electronic address:
Aims: Running exercise has demonstrated efficacy in the prevention and treatment of depression, yet the underlying mechanisms remain incompletely elucidated. Mitochondrial dysfunction and impaired mitophagy have been implicated in depression pathogenesis, while SIRT1 has been shown to play a critical role in both depression and mitochondrial regulation. Building on these established associations, this study aimed to investigate the antidepressant mechanisms of running exercise, with particular fucus on mitophagy regulated by SIRT1.
View Article and Find Full Text PDF