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Plants such as herbs, vegetables, fruits, and cereals are closely related to human life. Developing effective testing methods to ensure their safety and quantify their active components are of significant importance. Recently, nanomaterials with enzyme-like activity (known as nanozymes) have been widely developed in various assays, including colorimetric, fluorescence, chemiluminescence, and electrochemical analysis. This review presents the latest advances in analyzing phytochemicals and hazardous substances in plant samples based on nanozymes, including some active ingredients, organophosphorus pesticides, heavy metal ions, and mycotoxins. Additionally, the current shortcomings and challenges of the actual sample analysis were discussed.
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http://dx.doi.org/10.1186/s13020-024-01014-9 | DOI Listing |
Mikrochim Acta
September 2025
Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, Shenyang, 110016, Liaoning, People's Republic of China.
A novel dual-mode sensing system integrating a magnetic core-shell CuFeO/Cu/MnO nanozyme with a stimuli-responsive agarose-deep eutectic solvent hydrogel (DES-Aga) is reported. The nanozyme exhibits exceptional oxidase-like activity, characterized by a low Michaelis constant (K = 0.14 mM) and high catalytic efficiency (V = 1.
View Article and Find Full Text PDFBiomater Res
September 2025
Laboratory of Medical Imaging, The First People's Hospital of Zhenjiang, Zhenjiang 212001, P. R. China.
Mesoporous metal nanomaterials (MMNs) have gained interest in biomedicine for their unique properties, but their potential is limited by the predominance of spherical shapes and the neglect of morphological effects on biological activity, which hinders the reasonable evaluation of morphology-dependent enzyme-like activities and biological behaviors and its further biomedical applications. It is therefore imperative to find an effective and facile method to design and prepare MMNs with novel, well-defined morphologies. Herein, we fabricated 3 mesoporous platinum nanoenzymes including sphere, rod, and bipyramid topologies [Au@mesoPt sphere, Au@mesoPt rod, and Au@mesoPt bipyramid nanoparticles (NPs), respectively] via a facile atomic layer deposition method using gold NPs (Au NPs) as the templated cores and Pluronic F127 as a structure-directing agent.
View Article and Find Full Text PDFTalanta
September 2025
College of Pharmacy, Shanxi Medical University, Taiyuan, 030001, China. Electronic address:
Hydrogen peroxide (HO) is a key signaling molecule in tumor progression, making its real-time detection vital for elucidating the complex mechanisms underlying tumorigenesis. Herein, we report a rationally colorimetric sensing platform for rapid tumor screening, leveraging the bifunctional enzyme-like activity of a heterostructured h-NiO/CoO/C nanosphere. Notably, by activating electron structure reconstruction with abundant oxygen vacancies and utilizing a dual-non-precious-metal method, h-NiO/CoO/C nanosphere enhances catalytic performance beyond the limitations of single-non-precious-metal-doped nanomaterials (e.
View Article and Find Full Text PDFAnal Chem
September 2025
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
N-doped carbon nanomaterials (NCMs) have attracted significant interest as metal-free nanozymes for sensing due to their exceptional stability and biocompatibility. However, the controversial active sites and catalytic pathways severely hinder the application of NCM-based nanozymes. Here, postsynthetic modification methods have been developed to study the catalytic mechanism, including selective deactivation, chemical grafting, and surface doping.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2025
Department of Orthopaedics, Affiliated Hospital of Guilin Medical University, Guilin 541000, Guangxi, China.
Nanozymes, an emerging class of nanomaterials, demonstrate enzyme-like activities and have significantly broadened the enzyme mimicry field by extending it from organic to inorganic materials. Their affordability, multifunctionality, and enhanced stability under harsh conditions position them as potential replacements for natural enzymes in various applications. Nanozymes have demonstrated tremendous application potential in orthopedic diseases including osteoarthritis, osteoporosis, bone regeneration, bacteria-related infections, rheumatoid arthritis, and osteosarcoma.
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