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A new series of multivalent gold nanoparticle probes bearing different electrophilic groups were synthesized and their affinity labeling reactivities were evaluated. The dichlorotriazine group was identified as a useful protein-reactive label, allowing selective capture of a target protein at nanomolar probe concentrations.
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http://dx.doi.org/10.1039/d4ob01285b | DOI Listing |
Cell Rep Methods
September 2025
Lingang Laboratory, Shanghai 201306, China. Electronic address:
While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced a proteomic method termed affinity purification coupled proximity labeling-mass spectrometry (APPLE-MS), which combines the high specificity of Twin-Strep tag enrichment with PafA-mediated proximity labeling. This method achieves improved sensitivity while maintaining high specificity (4.
View Article and Find Full Text PDFJ Neurosci Methods
September 2025
European Laboratory for Non-linear Spectroscopy, via Nello Carrara 1, 50019 Sesto Fiorentino, Italy; National Institute of Optics -National Research Council (CNR-INO), 50125 Sesto Fiorentino, Italy. Electronic address:
Background: Tissue clearing techniques combined with light-sheet fluorescence microscopy (LSFM) enable high-resolution 3D imaging of biological structures without physical sectioning. While widely used in neuroscience to determine brain architecture and connectomics, their application for spinal cord mapping remains more limited, posing challenges for studying demyelinating diseases like multiple sclerosis. Myelin visualization in cleared tissues is particularly difficult due to the lipid-removal nature of most clearing protocols, and alternative immunolabeling approaches failed to reach satisfying results.
View Article and Find Full Text PDFJ Labelled Comp Radiopharm
September 2025
State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers, Beijing Key Laboratory of Research, Investigation and Evaluation of Radiopharmaceuticals, NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Departmen
A peptide-based, hypoxia-inducible factor-1 α (HIF-1α) specific PET tracer for tumor hypoxia imaging is reported. It was prepared with a rapid AlF labeling method with high stability. AlF-CLLFVY specifically binds to HIF-1α with high affinity and shows higher uptake in cells under hypoxia.
View Article and Find Full Text PDFMol Cell Proteomics
September 2025
Institute of Biotechnology, HiLIFE, Faculty of Medicine, University of Helsinki, Helsinki, Finland. Electronic address:
Structural proteomics has undergone a profound transformation, driven by the convergence of advanced experimental methodologies and computational innovations. Cutting-edge mass spectrometry (MS)-based approaches, including cross-linking MS (XL-MS), hydrogen-deuterium exchange MS (HDX-MS), and limited proteolysis MS (LiP-MS), now enable unprecedented insights into protein topology, conformational dynamics, and protein-protein interactions. These methods, complemented by affinity purification (AP), co-immunoprecipitation (co-IP), proximity labeling (PL), and spatial proteomics techniques, have expanded our ability to characterize the structural proteome at a systems-wide scale.
View Article and Find Full Text PDFAnal Chim Acta
October 2025
Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei, 230036, China. Electronic address:
Background: The excessive use of pesticide pollutants in agricultural production seriously threatens food safety. Traditional detection techniques are difficult to meet the detection requirements due to the complex sample pretreatment and high detection costs. The immunochromatography method (ICA) is simple to operate and fast, and is suitable for on-site rapid detection.
View Article and Find Full Text PDF