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http://dx.doi.org/10.1021/acscentsci.4c00940 | DOI Listing |
Analyst
September 2025
Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
Acute respiratory tract diseases (ARDs) are predominantly caused by viral infections, with one of their hallmark characteristics being multiple viral co-infections. Such multiple viral infections not only complicate therapeutic interventions but also lead to an increase in mortality rates. Most traditional bioassays, however, are limited to identifying a single type of virus, leading to missed diagnoses in samples with multiple respiratory pathogens.
View Article and Find Full Text PDFLab Chip
August 2025
Istituto di Scienze Applicate e Sistemi Intelligenti (ISASI), Via Campi Flegrei 34, 80078 Pozzuoli, NA, Italy.
Marine ecosystems are in the spotlight, because environmental changes are threatening biodiversity and ecological functions. In this context, microalgae play key ecological roles both in planktonic and benthic ecosystems. Consequently, they are considered indispensable targets for global monitoring programs.
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August 2025
MOE Key Laboratory of Intelligent Biomanufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
The aberrant expression of RNAs in ovarian cancer (OC) progression highlights their potential as clinical biomarkers. However, rapid and accurate quantification of these RNAs in biosamples remains a significant challenge. In this study, we develop a modular isothermal rolling circle amplification (RCA)-activated Cas12a loop-enhanced (MIRACLE) amplification method for circRNA and miRNA quantification without the need of reverse transcription.
View Article and Find Full Text PDFBiosens Bioelectron
December 2025
Beijing Key Laboratory of Microanalysis Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, PR China. Electronic address:
A sensitive analysis for self-carried biomarkers of tumor cells is of great significance for early tumor diagnosis and following treatment monitoring. In this work, open microfluidics combined with mass spectrometry for Epithelial cell adhesion molecule (EpCAM) analysis using DNA-mediated rolling circle amplification (RCA) was proposed. Following aptamer binding, the EpCAM signal was amplified and hybridized with a short DNA probe, which could be released by extracting with a denaturant agent and detected via mass spectrometry.
View Article and Find Full Text PDFAnal Chem
September 2025
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.
miRNAs regulate cancer progression and serve as both biomarkers and therapeutic targets in chemotherapy and gene therapy. Current analytical platforms lack the capacity to concurrently satisfy single-cell resolution and target specificity while maintaining high-throughput performance and cost-effectiveness. This limitation underscores the critical demand for innovative precision detection technologies.
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