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http://dx.doi.org/10.3389/fmicb.2024.1448900 | DOI Listing |
Front Syst Biol
August 2024
Microbial Ecology Laboratory, Microbiology, School of Biological and Chemical Sciences, College of Science and Engineering, University of Galway, Galway, Ireland.
Polyhydroxyalkanoates (PHA) are popular biopolymers due to their potential use as biodegradable thermoplastics. In this study, three aerobic sequencing batch reactors were operated identically except for their temperatures, which were set at 15 °C, 35 °C, and 48 °C. The reactors were subjected to a feast-famine feeding regime, where carbon sources are supplied intermittently, to enrich PHA-accumulating microbial consortia.
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June 2025
State Key Laboratory of Wheat Improvement, College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Livestock and poultry manure, as a significant organic resource, had an enormous annual production but a utilization rate of less than 50%. Improperly managed manure had become the primary source of agricultural non-point pollution, posing severe challenges to the ecological environment. Achieving efficient resource utilization of livestock manure was a critical step in promoting green agricultural development.
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January 2025
Department of Earth, Marine and Environmental Sciences, University of North Carolina, Chapel Hill, NC, United States.
Hydrothermal sediments host phylogenetically diverse and physiologically complex microbial communities. Previous studies of microbial community structure in hydrothermal sediments have typically used short-read sequencing approaches. To improve on these approaches, we use LoopSeq, a high-throughput synthetic long-read sequencing method that has yielded promising results in analyses of microbial ecosystems, such as the human gut microbiome.
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September 2024
Institute of Bioengineering, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
Front Microbiol
September 2024
Molecular Biology of Archaea, Faculty of Biology, University of Freiburg, Freiburg im Breisgau, Germany.
Introduction: Among hyperthermophilic organisms, protein localization is challenging due to the high growth temperatures that can disrupt proper folding and function of mostly mesophilic-derived fluorescent proteins. While protein localization in the thermophilic model archaeon has been achieved using antibodies with fluorescent probes in fixed cells, the use of thermostable fluorescent proteins for live imaging in thermophilic archaea has so far been unsuccessful. Given the significance of live protein localization in the field of archaeal cell biology, we aimed to identify fluorescent proteins for use in .
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