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Article Abstract
Background: Cannabidiol (CBD), as an important therapeutic property of the cannabis plants, is mainly produced in the flower organs. Auxin response factors (ARFs) are play a crucial role in flower development and secondary metabolite production. However, the specific roles of ARF gene family in cannabis remain unknown.
Methods: In this study, various bioinformatics analysis of genes were conducted using online website and bioinformatics, quantitative real time PCR technology was used to investigate the expression patterns of the gene family in different tissues of different cannabis varieties, and subcellular localization analysis was performed in tobacco leaf.
Results: In this study, 22 genes were identified and found to be unevenly distributed across 9 chromosomes of the cannabis genome. Phylogenetic analysis revealed that the ARF proteins were divided into 4 subgroups. Duplication analysis identified one pair of segmental/whole-genome duplicated , and three pairs of tandemly duplicated . Collinearity analysis revealed that two genes, and , were orthologous in both rice and soybean. Furthermore, subcellular localization analysis showed that was localized in the nucleus. Tissue-specific expression analysis revealed that six genes were highly expressed in cannabis male flowers, and among these genes, 3 genes were further found to be highly expressed at different developmental stages of male flowers. Meanwhile, correlation analysis between the expression level of genes and CBD content in two cultivars 'H8' and 'Y7' showed that the expression level of was negatively correlated with CBD content, while the expression levels of six genes were positively correlated with CBD content. In addition, most of genes were responsive to IAA treatment.
Conclusion: Our study laid a foundation for the further studies of CsARFs function in cannabis, and provides candidate genes for breeding varieties with high CBD yield in cannabis production.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188406 | PMC |
| http://dx.doi.org/10.3389/fpls.2024.1394337 | DOI Listing |
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