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Article Abstract
Six oxidosqualene cyclases (OSC1-OSC6) from were characterized for the biosynthesis of diverse triterpene scaffolds, including tetracyclic and pentacyclic triterpenes from the 2,3-oxidosqualene () and oxacyclic triterpenes from the 2,3:22,23-dioxidosqualene (). OSC1 showed high efficiency in the production of naturally rare (20)-epimers of oxacyclic triterpenes. Mutagenesis results revealed that the OSC1-F731G mutant significantly increased the yields of (20)-epimers compared to the wild type. Homology modeling and molecular docking elucidated the origin of the (20)-configuration in the epoxide addition step.
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