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http://dx.doi.org/10.3967/bes2024.036 | DOI Listing |
BMJ Open
September 2025
Microbiology Laboratory, Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.
Objectives: Globally, the circulation of influenza and other seasonal respiratory viruses changed dramatically during the COVID-19 pandemic. This study aims to determine the trends of acute respiratory infections (ARIs) caused by SARS-CoV-2, influenza A, influenza B and respiratory syncytial viruses (RSVs) in patients presenting to hospitals in the Lao People's Democratic Republic (PDR) (Laos).
Design: Prospective surveillance study.
Front Vet Sci
July 2025
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, China.
strain 2023 (CAPRV2023) has recently emerged as a significant pathogen responsible for substantial mortality in farmed golden pompano () across China, threatening the sustainability of the aquaculture industry. To address the urgent need for rapid and accurate diagnostics, we developed two TaqMan probe-based quantitative PCR (qPCR) assays targeting the viral G protein gene: a two-step qPCR assay and a one-step qPCR assay. The newly developed two-step qPCR assay demonstrated excellent performance, with a detection limit of 2 copies/μL and an amplification efficiency of 104.
View Article and Find Full Text PDFFront Microbiol
July 2025
Exotic Herbivore Disease Surveillance and Research Center, China Animal Health and Epidemiology Center, Qingdao, China.
Peste des petits ruminants (PPR) is a highly contagious disease caused by peste des petits ruminants virus (PPRV). PPRV is classified into four lineages based on the nucleocapsid (N) or fusion (F) genes. We established a TaqMan quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay using a pair of primers and a probe based on the phosphoprotein (P) gene.
View Article and Find Full Text PDFSci Rep
July 2025
Department of Chemistry, Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78464, Konstanz, Germany.
Reverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread applications in profiling gene expression, viral detection and the diagnosis of various diseases. Wellestablished methodologies use viral reverse transcriptases (RTs) to transcribe RNA to cDNA and thermostable DNA polymerases (DNA pols) to amplify the resulting target sequence by PCR.
View Article and Find Full Text PDFWorld J Gastroenterol
July 2025
Department of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
Background: Exosomal microRNAs (miRNAs) have emerged as promising biomarkers for cancer diagnosis due to their stability, tumor specificity, and accessibility. However, the diagnostic potential of serum exosomal miRNAs in metastatic pancreatic cancer remains underexplored.
Aim: To investigate the diagnostic potential of serum exosomal miRNAs in metastatic pancreatic cancer.