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Article Abstract

Meat discoloration starts at the interface between the bright red oxymyoglobin layer and the interior deoxymyoglobin layer. Currently, limited tools are available to characterize myoglobin forms formed within the sub-surface of meat. The objective was to demonstrate a needle-probe based single-fiber reflectance (SfR) spectroscopy approach for characterizing sub-surface myoglobin forms of beef psoas major muscles during retail storage. A 400-μm fiber was placed in a 17-gauge needle, and the assembly was inserted into the muscle at five depths of 1 mm increment and 1 cm lateral shift. Metmyoglobin content increased at all depths during display and content at 1 mm was greater compared to that of 2 to 5 mm depth. The a* values decreased (P < 0.05) during retail display aligning with the sub-surface formation of metmyoglobin. In summary, the results suggest that needle-probe SfR spectroscopy can determine interior myoglobin forms and characterize meat discoloration.

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http://dx.doi.org/10.1016/j.meatsci.2024.109439DOI Listing

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Article Synopsis
  • The study focuses on simplifying diffuse reflectance spectroscopy (DRS) to analyze myoglobin changes in meat through a non-contact method, enhancing the evaluation of meat discoloration.
  • The researchers introduced a straightforward forward model for DRS in a specific geometry (center-illuminated-area-detection) that significantly outperforms previous complex models, especially at high absorption levels.
  • By applying this model to various steaks over a week, findings on myoglobin transformations were consistent with results from traditional DRS methods, demonstrating the model's effectiveness in accurately evaluating meat quality.
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