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Meat discoloration starts at the interface between the bright red oxymyoglobin layer and the interior deoxymyoglobin layer. Currently, limited tools are available to characterize myoglobin forms formed within the sub-surface of meat. The objective was to demonstrate a needle-probe based single-fiber reflectance (SfR) spectroscopy approach for characterizing sub-surface myoglobin forms of beef psoas major muscles during retail storage. A 400-μm fiber was placed in a 17-gauge needle, and the assembly was inserted into the muscle at five depths of 1 mm increment and 1 cm lateral shift. Metmyoglobin content increased at all depths during display and content at 1 mm was greater compared to that of 2 to 5 mm depth. The a* values decreased (P < 0.05) during retail display aligning with the sub-surface formation of metmyoglobin. In summary, the results suggest that needle-probe SfR spectroscopy can determine interior myoglobin forms and characterize meat discoloration.
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http://dx.doi.org/10.1016/j.meatsci.2024.109439 | DOI Listing |
Org Lett
September 2025
School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.
Current research on artificial aldolases predominantly centers on aldehyde substrates with relatively limited exploration of ketone substrates. Here, we report the creation of a novel artificial aldolase based on apo-myoglobin by embedding an organocatalytic secondary amine cofactor in the protein's distal pocket. The designer enzyme exhibits good to excellent enantioselectivities (up to 97.
View Article and Find Full Text PDFFood Chem
August 2025
Key Laboratory of Characteristics Agricultural Product Processing and Quality Control (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; Key Laboratory for Food Nutrition a
We investigated the effects and mechanisms of two sodium chloride concentrations (0.3 and 0.6 M) and cumin essential oil concentrations (CEO, 0.
View Article and Find Full Text PDFJ Am Chem Soc
August 2025
Department of Chemistry, University of Texas at Austin, Austin, Texas 78712, United States.
Heme proteins are essential metalloproteins with diverse biological roles, and these functions are facilitated by the heme's ability to adopt multiple oxidation states, with Fe(II), Fe(III), and Fe(IV) being the most commonly observed. While highly reduced heme states beyond Fe(II) have been studied in synthetic complexes, their presence and characterization in native hemoproteins have remained largely unexplored. In this study, we report a full conversion of sperm whale myoglobin (Mb) to an ultrareduced state through chemical reduction under physiologically relevant conditions.
View Article and Find Full Text PDFMeat Sci
November 2025
Food Safety and Functionality, IRTA, Finca Camps i Armet s/n, 17121 Monells, Catalonia, Spain. Electronic address:
As an alternative to adding pure nitrite sources to dry-fermented sausages, we examined three different strategies involving the addition of two meat-associated coagulase-negative staphylococci (CNS) showing nitric-oxide synthase (NOS) activity, a porcine liver extract rich in the pigment zinc protoporphyrin (ZnPP), and a polyphenol-rich ingredient (NATPRE T-10 CUR HT) alone or in combination with the other strategies. We studied the colour development of nine dry-fermented sausages originating from a meat batter control without nitrite (CTRL-), control with nitrite (CTRL+), inoculated with Staphylococcus equorum (L33), inoculated with Staphylococcus saprophyticus (L49), formulated with a ZnPP-rich extract (ZnPPEx), formulated with NATPRE alone (CTRL-/NATPRE) or in combination with the other strategies (L33/NATPRE, L49/NATPRE, ZnPPEx/NATPRE, respectively). The colour was affected by the treatment, ripening period, and their interaction (P < 0.
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