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The high toxicity and occurrence of ochratoxin A (OTA) in grains and foods has been a growing concern due to the impacts on health and the economy in many countries. In this sense, simplified devices with high sensitivity and specificity for local monitoring are enthusiastically pursued. In this work, we report for the first time the detection of ochratoxin A in coffee samples using a spoon-shaped waveguide immunosensor. The biosensor was built with the surface of the spoon-shaped waveguide covered by a 60 nm layer of gold to enable the SPR phenomenon. The measurements indicated a linear relationship between the change in the SPR phenomenon values and the OTA concentration in the range from 0.2 ppt to 5 ppt. When analyzed in coffee samples, the biosensor was highly selective and did not suffer matrix interference. The developed biosensor represents a promising analytical device for coffee quality analyses, as it is portable, simple, and suitable for onsite detection of target analytes.
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http://dx.doi.org/10.1016/j.talanta.2024.125648 | DOI Listing |
Cureus
August 2025
Prosthodontics, Kerala University of Health Sciences, Thrissur, IND.
Background and objectives With the continuous presence of microflora, saliva, and frequent intake of coloured food, the colour stability of any aesthetic material may become compromised. Hence, the present study was conducted to evaluate the influence of tea, coffee, and turmeric solutions on the colour stability of commercially available heat-cured and autopolymerizing denture base acrylic resins as well as a soft lining material. Methods Twenty-four rectangular samples measuring 20 mm × 15 mm × 2 mm were prepared for each type of test material.
View Article and Find Full Text PDFCan J Microbiol
September 2025
Universidad de Costa Rica, School of Microbiology & Center for Research in Tropical Diseases (CIET), San José, Costa Rica;
Coffee plants and beans are prone to fungal contamination that pose health risks to consumers by producing mycotoxins like ochratoxin A (OTA). Thus, the present study aimed to analyze the mycobiota of Costa Rican coffee beans, focusing on potentially ochratoxigenic species and their in vitro susceptibility patterns to antifungal agents. Fungal isolates were obtained from cherry, green, and roasted coffee beans from Costa Rica; they were identified by morphology, MALDI-TOF technology, and sequencing.
View Article and Find Full Text PDFMicrobiologyopen
October 2025
Department of Biochemistry, Faculty of Science and Technology, Chiromo Campus, Off Riverside Drive, University of Nairobi, Nairobi, Kenya.
Alkaline pectinases are in demand in industrial processes that require the degradation of plant pectins at high pH, for example, removal of pectin stains from fabrics, cutlery, and porcelain; treatment of pectic wastewater; fermentation of coffee, tea, and cocoa; manufacture of poultry and animal feeds, and processing of textiles, and so forth. The present study aimed to (a) screen four alkaliphilic microbial isolates, previously obtained from samples collected around Lake Bogoria (soda lake), Baringo County, Kenya, for alkaline pectinases, and (b) characterize the pectinase-producers. The screening data revealed that all the isolates were pectinase producers, exhibiting catalytic activities that ranged from 1.
View Article and Find Full Text PDFAnal Chim Acta
November 2025
NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical University, Haikou, Hainan, 571199, China. Electronic address:
Background: While paper-based colorimetric assays have seen significant progress in recent years, persistent challenges including the coffee-ring effect and infiltration effect continue to affect the color uniformity of detection results, leading to decreased sensitivity and accuracy of the detection. Recent advancements in suppressing these two effects mainly depend on chemical modification of cellulose fibers or application of specific functional coatings. However, the former's complex procedures impede large-scale implementation, while the latter's non-cellulosic additives risk unpredictable interactions with analytes or interference in colorimetric reactions.
View Article and Find Full Text PDFPlant Foods Hum Nutr
September 2025
REQUIMTE/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
The growing interest in prebiotic ingredients has led to the valorization of agri-food by-products, such as coffee silverskin, known for its richness in dietary fiber and health-promoting compounds. This study evaluated the impact of in vitro simulation of gastrointestinal digestion on the chemical composition (carbohydrates, caffeine, and chlorogenic acids) and prebiotic potential (probiotic growth, organic acid production, pH, and antioxidant activity) of milled coffee silverskin. The results show stability of polysaccharides during digestion, while caffeine and 5-caffeoylquinic acid were partially released into the bioaccessible fraction.
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