Changes in gut microbiota signatures associated with the epidemiological dynamics of wild European rabbits facing haemorrhagic disease outbreaks.

The new variant of rabbit haemorrhagic disease virus (RHDV2 or RHDVb) is responsible for a lethal, emerging infectious disease in several species of lagomorphs, and is globally threatening wild rabbit populations. It is known that the gut microbiota plays a crucial role in modulating host health, including immune responses and disease susceptibility. We hypothesize potential association of gut microbiota with the epidemiological dynamics of RHDV2 outbreaks that may provide key insights into how this lethal, emerging pathogen impacts wild rabbit populations.

From pan-active to parasite-selective antiparasitic agents: A scaffold hopping approach.

Vector-borne parasitic diseases (VBPDs) represent a major global public health concern, with human African trypanosomiasis (HAT), Chagas disease, leishmaniasis, and malaria collectively threatening millions of people, particularly in developing regions. Climate change may further influence their transmission and geographic spread, increasing the global burden. As drug resistance continues to rise, there is an urgent need for novel therapeutic agents to expand treatment options and limit disease progression.

Cool Solutions in Hot Times: The Case for Digital Health in Heatwave Action Plans.

This viewpoint highlights the critical need for proactive and strategic integration of digital health tools into heat-health action plans (HHAPs) across Europe. Drawing insights from the digital health surge during the COVID-19 pandemic and recent heat-related health impacts, we identify response gaps and suggest specific strategies to strengthen current plans. Key recommendations include leveraging mobile health communication, expanding telemedicine usage, adopting wearable health monitoring devices, and using advanced data analytics to improve responsiveness and equity.

Forms of interventions for problematic usage of the internet: A scoping review.

Background And Aims: This scoping review aims to identify current forms of interventions for Problematic Usage of the Internet (PUI) to inform more effective intervention and policy-making initiatives grounded in robust empirical evidence.

Methods: The search was conducted in the PubMed, Scopus, and PsycINFO databases until October 12, 2024. Empirical research presenting data on interventions for PUI and written in English was included without restrictions of age groups, types of interventions, or types of PUI.

Impact of In vitro Gastrointestinal Digestion on the Chemical Composition and Prebiotic Potential of Coffee Silverskin.

The growing interest in prebiotic ingredients has led to the valorization of agri-food by-products, such as coffee silverskin, known for its richness in dietary fiber and health-promoting compounds. This study evaluated the impact of in vitro simulation of gastrointestinal digestion on the chemical composition (carbohydrates, caffeine, and chlorogenic acids) and prebiotic potential (probiotic growth, organic acid production, pH, and antioxidant activity) of milled coffee silverskin. The results show stability of polysaccharides during digestion, while caffeine and 5-caffeoylquinic acid were partially released into the bioaccessible fraction.

Electrophysiological responses of the clam (Ruditapes decussatus) osphradium to amino acids and alarm cues.

Chemical sensing of the surrounding environment is crucial for many aspects of bivalve biology, such as food detection and predator avoidance. Aquatic organisms strongly depend on chemosensory systems; however, little is known about chemosensory systems in bivalves. To understand how the carpet shell clam (Ruditapes decussatus) senses its surrounding chemical environment, we used an electrophysiological technique - the electro-osphradiogram - to assess the sensitivity of the osphradium to different putative odorants (amino acids, bile acids) and odours (predator-released cues and signals from con- and heterospecific bivalves).

Lipidomic Signature of Abdominal Aortic Aneurysm and Peripheral Artery Disease.

Vascular diseases are powerful predictors of cardiovascular mortality, but they are typically under-recognized and undertreated. There is no effective treatment for either abdominal aortic aneurysm (AAA) or peripheral artery disease (PAD). Lipids are key molecules in cardiovascular diseases and good candidates for diagnosis, monitoring, and risk prediction; nonetheless, there is very limited information on the lipidomic profile of patients with AAA and PAD.

Point-of-care ultrasound can make the difference in patients with heart failure at primary care.

Background: Primary healthcare centers (PHC) play a pivotal role in the first-line management of patients with diabetes and hypertension, major risk factors for heart failure (HF) development. Point-of-care cardiac ultrasound (POCUS), integrated as an extension of the physical examination, holds significant potential to enhance diagnostic accuracy and clinical management in this setting.

Objectives: Evaluate the impact of POCUS on clinical decision-making in patients with HF and at risk of developing HF in PHC and compare POCUS findings with clinical assessment alone, conventional echocardiography, and electrocardiogram results.

Non-invasive bladder cancer detection: Identification of a urinary volatile biomarker panel using GC-MS metabolomics and machine learning.

Early detection of bladder cancer (BC) remains a major clinical challenge due to the limitations of current diagnostic methods, which are often invasive, expensive, or insufficiently sensitive, particularly for early-stage disease. Metabolomics approaches, when integrated with machine learning (ML) techniques, offer a powerful platform for identifying novel, non-invasive biomarkers. In this study, urinary volatile organic compounds (VOCs) were analysed from 87 BC patients and 90 age- and sex-matched cancer-free controls using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS).

The Impact of War on Asthma, a Systematic Review and Meta-Analysis: An EAACI Task Force Report.

Wartime events have been followed by an increase in asthma prevalence, which is believed to result from a combination of environmental hazards and psychological trauma. This systematic review and meta-analysis aimed to investigate this relationship by pooling available data on various wartime exposures, such as occupational, environmental, and psychological factors. MEDLINE, Scopus, and Cochrane databases were searched for articles that measure the effect of war-related exposures on asthma.

Assessment of Lumbar Vertebrae L1-L7 and Proximal Femur Microstructure in Sheep as a Large Animal Model for Osteoporosis Research.

Sheep have been widely used as a model for osteoporosis research. This study aimed to characterise changes in microstructure and composition in lumbar vertebrae L1-L7 and the proximal femur after implementation of a bone loss induction protocol (in this species). A sham control and experimental group (glucocorticoid-treated ovariectomized sheep) were used ( = 6/group), with a study duration up to the 24th postoperative week.

Unraveling the Systemic and Local Immune Response of Rainbow Trout () to the Viral Hemorrhagic Septicemic Virus.

Viral outbreaks have caused significant mortality and economic losses in aquaculture, highlighting the urgent need for effective therapies and a deeper understanding of antiviral and immune mechanisms in key species. This study investigates the constitutive and virus-induced antiviral responses in juvenile rainbow trout () following infection with viral hemorrhagic septicemia virus (VHSV). Trout (30 g) were infected by immersion with VHSV (TCID = 10 mL) for two hours.

Accurate Analysis of the Pitch Pulse-Based Magnitude/Phase Structure of Natural Vowels and Assessment of Three Lightweight Time/Frequency Voicing Restoration Methods.

This paper addresses two challenges that are intertwined and are key in informing signal processing methods restoring natural (voiced) speech from whispered speech. The first challenge involves characterizing and modeling the evolution of the harmonic phase/magnitude structure of a sequence of individual pitch periods in a voiced region of natural speech comprising sustained or co-articulated vowels. A novel algorithm segmenting individual pitch pulses is proposed, which is then used to obtain illustrative results highlighting important differences between sustained and co-articulated vowels, and suggesting practical synthetic voicing approaches.

Response to biologics along a gradient of T2 involvement in patients with severe asthma: a data-driven biomarker clustering approach.

Background: Asthma with low levels of T2-biomarkers is poorly understood.

Objective: To characterize severe asthma phenotypes and compare pre- to post-biologic change in asthma outcomes along a gradient of T2-involvement.

Methods: This was a registry-based, cohort study including data from 24 countries.

The Impact of Glenohumeral Rotational Asymmetries on the Risk of Shoulder Pain in Volleyball Players: A Cross-Sectional Study.

Background: Shoulder rotational imbalances are common adaptations in volleyball players due to repetitive overhead movements. This study investigates the relationship between shoulder rotational asymmetries and the risk of shoulder pain in professional and semi-professional volleyball players.

Methods: A cross-sectional study was conducted on 84 volleyball players, divided into groups with and without history of shoulder pain (n=29 and n=55, respectively).

Testicular germ cell tumors and infertility: Exploring epigenetic dysregulation in the journey of the male germ cell towards new biomarkers and therapeutics.

The development of male germ cells comprises a series of differentiation events that culminate in the formation of spermatozoa. From the primordial germ cell (PGC) to the spermatid, an array of epigenetic events take place in different stages of life. Disruptions in these mechanisms due to external factors can ultimately lead to decreased fertility and to testicular germ cell tumors (TGCTs).

Adherence to Clinical Practice Guidelines for Management of Epithelial Precancerous Conditions and Lesions in the Stomach in Europe.

Background And Aims: The first international guideline for managing preneoplastic conditions of the stomach (MAPS I) was published in 2012, followed by an update (MAPS II) in 2019. As adherence to these guidelines remains uncertain, we evaluated adherence by comparing the management of preneoplastic gastric conditions before and after the introduction of MAPS I and II in selected European centers.

Methods: Patients data were retrieved from nine endoscopy units in seven European countries during three periods: pre-MAPS I (2010/2011), post-MAPS I (2017/2018), and post-MAPS II (2022/2023).

The role of pharmacists in the management of patients with atrial fibrillation: A systematic review and meta-analysis.

Background: Pharmacist-led interventions have demonstrated benefits across various medical conditions; however, their impact on atrial fibrillation (AF) remains unexplored. This study aims to synthesize the available evidence regarding the pharmacist's role in AF management.

Methods: A systematic review with searches in PubMed, Scopus, and Web of Science was performed (PROSPERO: CRD42025647848).

Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids.

The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish health and product quality. Polychaetes represent a promising alternative. While marine polychaetes show complete LC-PUFA biosynthetic pathways involving elongases (Elovl), front-end desaturases (Fed), and methyl-end desaturases (ω des), freshwater species remain poorly studied.

Control of Allergic Rhinitis and Asthma Test for Children (CARATkids): A systematic review and meta-analysis of its measurement properties.

Control of Allergic Rhinitis and Asthma Test for Children (CARATkids) is the first patient-reported outcome measure (PROM) designed to assess both allergic rhinitis and asthma simultaneously in children aged 6 to 12 years. CARATkids has been validated in several languages and countries, highlighting the need for a review of its psychometric properties. This study aims to evaluate the measurement properties of CARATkids.

Shipping emissions forecast in the Iberian Peninsula for 2050 impact on air quality, mortality and related costs.

The International Transport Forum projected that global demand for transport could triple by 2050, with shipping demand likely increasing even more rapidly. However, most scenarios for the next 20-30 years suggest that current regulatory policies may be insufficient to offset the rise in traffic. Thus, this study evaluates the impacts of shipping emissions on air quality, health, and external costs in the Iberian Peninsula, analysing past (2017), current (2022), and projected future (2050) scenarios.

Conducting multicenter simulation-based experimental research: lessons drawn from the Q European project.

Multicenter research designs are widely recognized for enhancing the generalizability and real-world applicability of findings across diverse healthcare settings. However, conducting experimental studies across international sites presents substantial methodological, logistical, and operational challenges. Quality CPR was a European multicenter simulation-based experimental study conducted across academic institutions in Portugal, Germany, and Finland.

Herpes Simplex Virus Type-1 Infection as a Trigger for Pemphigus Vulgaris.

Pemphigus vulgaris (PV) is a mucocutaneous autoimmune disorder that can be challenging to diagnose and treat. We discuss a clinical case of a patient with a previous medical history of autoimmune gastritis and vitiligo who presented with painful oral ulceration but was devoid of dermatological manifestations. Immunoglobulin M (IgM) antibodies and polymerase chain reaction (PCR) amplification of Herpes simplex virus type 1 (HSV-1) were detected from the base of the ulcers.