Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
is found worldwide and causes chronic parasitism in its human hosts. We developed a diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection. Using 18S rRNA plasmid DNA, the assay demonstrates a detection limit of 10 copies /μL (~1.7 genome equivalents) and 100% analytical specificity. Testing in field samples showed 95% clinical sensitivity and 88% specificity compared to qPCR. Total assay time was 35 minutes. Combined with simplified DNA extraction methods, the assay has potential for future field-deployable point-of-care use to detect a parasite species that remains largely undiagnosed.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543045 | PMC |
| http://dx.doi.org/10.1101/2023.02.26.23286371 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual-functional hydrochar via hydrothermal carbonization for norfloxacin removal: Fractal adsorption kinetics and mechanism elucidation.
Sci Total Environ
September 2025
Laboratoire Physico-Chimie des Matériaux, Substances Naturelles et Environnement, Faculty of Sciences and Techniques, Abdelmalek Essaâdi University, Tangier, Morocco.
Escalating concentrations of norfloxacin (NFX) in surface and wastewaters demand sustainable remediation strategies. In this study, dual-functional hydrochars were synthesized from argan nut shells (ArNS) via hydrothermal carbonization (HTC), with process conditions optimized by varying temperature (150-200 °C) and residence time (2-6 h). Among the materials, H1:5@150-4-prepared at 150 °C for 4 h with a biomass-to-water ratio of 1:5-exhibited the best performance, achieving a monolayer NFX adsorption capacity of 27.
View Article and Find Full Text PDFHydrogen Storage Law and Nanoscale Occurrence Mechanism of Anthracite Containing Depleted CH: Insights from Experiment and Molecular Simulation.
Langmuir
September 2025
School of Resources and Safety Engineering, Chongqing University, Chongqing 400044, China.
Hydrogen energy is pivotal for driving sustainable development and achieving deep decarbonization; yet, its storage remains a significant challenge. Notably, depleted methane reservoirs can serve as a promising large-scale solution for underground hydrogen storage (UHS). Based on adsorption experiments, Monte Carlo and molecular dynamics methods, the adsorption behavior of H and CH in anthracite and the applicability of five models were discussed.
View Article and Find Full Text PDFCu(II) Removal from Aqueous Samples Using In Situ Functionalized Silica with Novel Synthesized Formazan: Synthesis, Characterization, Kinetic and Isothermal Studies.
ACS Omega
September 2025
Department of Chemistry, Faculty of Science, Dokuz Eylul University, Izmir 35160, Turkey.
A novel silica-based sorbent, silica-carbazole-formazan (Si-Carb-Formazan), was synthesized through in situ functionalization with a newly prepared carbazole formazan derivative to remove Cu-(II) ions from aqueous solutions efficiently. The sorbent was characterized using techniques such as FTIR, SEM, TGA, and XPS, which revealed a porous structure with a high surface area and excellent thermal stability. Batch adsorption experiments analyzed the influence of various factors on the sorbent's performance, demonstrating its high efficiency.
View Article and Find Full Text PDFRapid C Solid-State Quantitative NMR Method for Multiple Physical and Chemical Analyses of Cocoa-Based Products: Proof of Concept.
Anal Chem
September 2025
Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, São Paulo 13560-970, Brazil.
Chocolates and other cocoa products represent a multibillion-dollar industry that has faced significant price increases, largely due to a surge in cocoa plant diseases linked to climate change. One potential solution for mitigating cocoa prices involves the use of cocoa butter equivalents, substitutes, or replacers. Consequently, a rapid method for simultaneously determining multiple properties of cocoa derivatives can serve as a valuable tool for research and development of new products, quality control, and regulatory agencies to ensure compliance with cocoa product standards.
View Article and Find Full Text PDFAdvances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.
Anal Sci
September 2025
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, People's Republic of China.
Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections.
View Article and Find Full Text PDF