Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Glycinin is one of the most nutritious ingredients in soybean, but it is also an allergen that can cause allergic reactions in humans and animals, and even endanger life in severe cases. Therefore, it is imperative to develop a rapid and ultrasensitive detection method for glycinin. In order to achieve this goal, this experiment combined surface-enhanced Raman spectroscopy (SERS) technology with an economical, simple, fast and easy-to-carry immunochromatographic test strip, and successfully constructed an efficient sandwich immunochromatographic test strip. The immunoprobe of the test strip was covalently coupled by gold nanostars (AuNSs), Raman molecule 4-aminothiophenol (4-PATP) and rabbit polyclonal antibody. In this experiment, a gold nanostar immunochromatographic test strip for detecting glycinin was established. The detection limit of the test strip was 0.23 ng / mL, the recovery rate was 91.5-96.6 %, and the coefficient of variation was 1.61-6.15 %. In addition, the test strip had no cross reaction with whey protein, wheat protein, peanut protein, sesame protein and β-conglycinin, indicating that the detection method had good specificity and the ability to avoid false positive results. Hence, this experiment successfully prepared a rapid and sensitive test strip for glycinin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.saa.2023.123439 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Smart and user-friendly food quality monitoring using a red-emitting fluorescent probe for volatile primary amine detection.
Talanta
September 2025
Karamanoglu Mehmetbey University, Kamil Ozdag Science Faculty, Department of Chemistry, Karaman, 70100, Turkey.
Biogenic amines (BAs) are organic nitrogen compounds formed through microbial decarboxylation of amino acids during food spoilage and biological metabolism. Therefore, the development of rapid, selective, and cost-effective detection strategies for BAs is significant for ensuring food safety and quality. In this study, a new dicyanoisophorone-based fluorescent probe (IPC) was developed, capable of fluorescence detection of aliphatic primary amines (e.
View Article and Find Full Text PDFClimate, Rotation, and Tillage Impacts on Soybean Yield Gains in a 50-Year Experiment.
Glob Chang Biol
September 2025
Department of Agronomy, Purdue University, West Lafayette, Indiana, USA.
Understanding how interactive management practices and climatic behavior influence soybean [Glycine max (L.) Merr.] productivity is imperative to inform future production systems under changing climate.
View Article and Find Full Text PDFSmart Lateral Flow Strip Biosensor for Visual and Portable Sensing of Antibiotic Residues Based on Cascaded Logic Circuits and DNA Biocomputations.
Anal Chem
September 2025
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
A series of molecular logic gates with multiple biocomputing capabilities have been successfully fabricated by using four antibiotic residues [tetracycline (TET), chloramphenicol (CHL), kanamycin (KAN), and streptomycin (STR)] as inputs. The lateral flow strip biosensor was utilized to realize the visual and portable sensing of logic events. Four basic logic gates (OR, AND, XOR, and INHIBIT) and three cascade logic circuits (OR-INHIBIT-AND, 3AND-OR, and XOR-INHIBIT-OR-AND) were constructed.
View Article and Find Full Text PDFHollow polydopamine for the enhanced ultrasensitive dual-modal immunochromatographic detection of Salmonella typhimurium in dairy products via machine learning.
Mikrochim Acta
September 2025
College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Salmonella typhimurium (S. typhimurium) A dual-mode colorimetric/photothermal immunochromatographic strip (ICS) employing hollow polydopamine nanoparticles (h-PDA) is reported for the ultrasensitive detection of Salmonella typhimurium (S. typhimurium).
View Article and Find Full Text PDFThe Impact of Two Cordless Retraction Materials on Inflammatory Biomarkers Interleukin-1 Beta and Tumor Necrosis Factor-Alpha in Gingival Crevicular Fluid: An In-Vivo Study.
Cureus
August 2025
Department of Orthodontics, Kothiwal Dental College and Research Centre, Moradabad, IND.
Introduction: This study aimed to evaluate and compare the inflammatory responses of two cordless gingival retraction systems by assessing the levels of interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) in gingival crevicular fluid (GCF). The objectives were to measure IL-1β and TNF-α levels before and after retraction using kaolin-based and polyvinyl acetate-based cordless gingival retraction materials at baseline, 1 h, and 24 h, and to compare the inflammatory profiles of these materials.
Materials And Methods: This in-vivo comparative study used a split-mouth design, with each of the 10 enrolled patients serving as their own control by receiving both kaolin-based paste (Traxodent®, Premier Dental Products, Plymouth Meeting, PA, USA) and polyvinyl acetate-based strip (Merocel®, Medtronic, Minneapolis, MN, USA) on different endodontically treated teeth requiring full-coverage crowns, thereby minimizing interindividual variability.