Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Cysteine-rich secretory proteins (C), antigen 5 (A), and pathogenesis-related 1 proteins (P) comprise widespread CAP superfamily proteins, which have been proven to be novel virulence factors of mammalian pathogenic fungi and some plant pathogens. Despite this, the identification and function of CAP proteins in more species of plant pathogens still need to be studied. This work presents the identification and functional analysis of CAP superfamily proteins from f. sp. (), an important fungal pathogen that causes wheat stripe rust on wheat worldwide. A total of six genes were identified in the genome, designated as -. Five PsCAP proteins, including PsCAP1, PsCAP2, PsCAP3, PsCAP4, and PsCAP5, have N-terminal signal peptides secreted with the yeast signal sequence trap assay. Single-nucleotide polymorphism (SNP) analysis indicated that they showed a low level of intraspecies polymorphism. The expression abundance of genes at different infection stages was detected by RT-qPCR, and most of them were highly expressed during infection on wheat and also sexual reproduction on barberry (). Noticeably, the silencing of these six genes by BSMV-mediated HIGS indicated that , , and contribute significantly to infection in wheat. These results indicate that PsCAP proteins may act as virulence factors during infection, which also provides insights into pathogenicity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381272 | PMC |
| http://dx.doi.org/10.3390/jof9070734 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of Cross-Linked Elastomer Networks by Thermal Desorption/Pyrolysis Direct Analysis in Real-Time Mass Spectrometry (TDPy-DART-MS).
Anal Chem
September 2025
Department of Chemistry, The University of Akron, Akron, Ohio 44325, United States.
Tires are complex polymeric materials composed of rubber elastomers (both natural and synthetic), fillers, steel wire, textiles, and a range of antioxidant and curing systems. These constituents are distributed differently among the various tire parts, which are classified based on their function and proximity to the rim. This study presents a rapid and sensitive approach for the characterization of tire components using mild thermal desorption/pyrolysis (TDPy) coupled to direct analysis in real-time mass spectrometry (DART-MS).
View Article and Find Full Text PDFBackground-Free Rolling Circle Amplification for SERS Bioassay Using a Chimeric Hairpin-Integrated CRISPR/Cas12a System.
Anal Chem
September 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361
Rolling circle amplification (RCA) has revolutionized nucleic acid detection owing to its isothermal simplicity. However, over two decades of clinical application have been hampered by off-target amplification and incompatibility with double-stranded DNA (dsDNA). Herein, a strategy, specifically cleavage of rationally designed DNA/RNA chimeric hairpin preprimer by dsDNA-targeted CRISPR/Cas12a to rlease ssRNA for initiating RCA (SCOPE-RCA), is proposed for nucleic acid identification of African swine fever virus (ASFV).
View Article and Find Full Text PDFEye Drop Instillation Success and Hand Function in Adults with Glaucoma: A Pilot Study.
Ophthalmol Glaucoma
September 2025
Department of Ophthalmology and Visual Sciences, University of Michigan W.K. Kellogg Eye Center, Ann Arbor, Michigan. Electronic address:
Purpose: To investigate hand function and eye drop instillation success in adults with and without glaucoma.
Design: Cross-sectional pilot study.
Subjects: Adults aged ≥ 65 years with glaucoma who use eye drops daily and adults aged 65+ without glaucoma who do not regularly use eye drops.
A phylogenetic approach to prioritising crop wild relatives in Brassiceae (Brassicaceae) for breeding applications.
Ann Bot
September 2025
Royal Botanic Gardens, Kew, Richmond, Research department, Surrey, TW9 3AE, UK.
Background And Aims: Crop wild relatives (CWRs) are key resources for enhancing agricultural resilience, providing genetic traits that can improve pest resistance, abiotic stress tolerance, and nutritional composition in domesticated crops. Within the mustard family (Brassicaceae) this is especially significant in the Brassiceae tribe, which includes economically important genera for agriculture such as Brassica and Sinapis. However, while breeding programmes have historically focused on major crops within this tribe, the potential of their wild relatives, particularly for underutilised and minor crops, remains insufficiently explored.
View Article and Find Full Text PDFIdentification and Exploration of Novel B Cell Infiltration-Related Biomarkers in Endometriosis.
Am J Reprod Immunol
September 2025
Department of Laboratory Animal Science, Kunming Medical University, Kunming, China.
Objective: To explore B cell infiltration-related genes in endometriosis (EM) and investigate their potential as diagnostic biomarkers.
Methods: Gene expression data from the GSE51981 dataset, containing 77 endometriosis and 34 control samples, were analyzed to detect differentially expressed genes (DEGs). The xCell algorithm was applied to estimate the infiltration levels of 64 immune and stromal cell types, focusing on B cells and naive B cells.