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Anaerobic digestion (AD) is promising for treating high-strength wastewater. However, the effect of operational parameters on microbial communities of AD with sulfate is not yet fully understood. To explore this, four reactors were operated under rapid- and slow-filling modes with different organic carbons. Reactors in the rapid-filling mode generally exhibited a fast kinetic property. For example, the degradation of ethanol was 4.6 times faster in ASBR than in ASBR, and the degradation of acetate was 11.2 times faster in ASBR than in ASBR. Nevertheless, reactors in the slow-filling mode could mitigate propionate accumulation when using ethanol as organic carbon. Taxonomic and functional analysis further supported that rapid- and slow-filling modes were suitable for the growth of r-strategists (e.g., Desulfomicrobium) and K-strategists (e.g., Geobacter), respectively. Overall, this study provides valuable insights into microbial interactions of AD processes with sulfate through the application of the r/K selection theory.
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http://dx.doi.org/10.1016/j.biortech.2023.129431 | DOI Listing |
MethodsX
December 2025
Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal-132001, India.
In vitro simulation of rumen fermentation is critical for improving feed efficiency, assessing dietary interventions, and supporting methane mitigation strategies in ruminant production systems. However, existing fermentation platforms are often expensive, technically complex, or poorly suited for long-term microbial viability under near-rumen conditions-especially in resource-limited settings. This study presents the development and validation of a modular, low-cost engineered to replicate key physiological parameters of the rumen, including temperature control (39-40 °C), continuous buffering via artificial saliva infusion, anaerobic regulation, and simulated motility through mixing pumps.
View Article and Find Full Text PDFBMC Vet Res
September 2025
Department of Poultry Production, Faculty of Agriculture, Fayoum University, Fayoum, 63514, Egypt.
This study investigated the impact of dietary zeolite supplementation on growth, cecal microbiota and digesta viscosity, digestive enzymes, carcass traits, blood constituents, and antioxidant parameters of broilers. A completely randomized design was used with 240 one-day-old broiler chicks randomly assigned to three dietary treatments (0%, 1.5%, and 3% zeolite as a feed additive) with four replicates of 20 chicks each.
View Article and Find Full Text PDFJ Environ Manage
September 2025
Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China. Electronic address:
Large-scale anaerobic treatment involves a high risk of antibiotic pollution in anaerobically digested (AD) biosolids, which hinders the efficient utilization of farmland AD biosolids. Herein, a process for the in situ removal of antibiotics from AD biosolids using ethylenediaminetetraacetic acid disodium salt dihydrate as the release agent synergized with sodium persulfate oxidation is reported. The developed process was used to remove antibiotics from actual AD biosolids.
View Article and Find Full Text PDFWater Res
August 2025
Westlake Laboratory of Life Sciences and Biomedicine, Center for Infectious Disease Research, School of Life Sciences, Westlake University, Hangzhou 310024, China; Zhejiang Provincial Key Laboratory of Intelligent Low-Carbon Biosynthesis, Research Center for Industries of the Future, School of Engin
Livestock wastewater is a critical reservoir of antibiotic resistance genes (ARGs) that poses significant public health risks. This study comprehensively evaluated the seasonal dynamics and associated risks of ARGs in a full-scale livestock wastewater treatment plant using an integrated metagenomic and metatranscriptomic approach. The results showed that untreated livestock wastewater harbored high abundance (4.
View Article and Find Full Text PDFBioresour Technol
September 2025
State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing 210023, China. Electronic address:
Sulfur-modified nanoscale zero-valent iron (S-nZVI) has emerged as a promising additive for enhancing anaerobic treatment of refractory wastewater. However,its long-term effectiveness and role in toxic shock resistance remain unclear. Herein, S-nZVI was first applied to continuous-flow anaerobic reactors treating wastewater containing 2,4-dichlorophenol (2,4-DCP).
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