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Article Abstract
In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for culture of fluorescent monocytic THP-1 cells and chemotaxis plate preparation with HUVEC monolayers. We then detail real-time analysis using the IncuCyte® S3 live-cell imaging system, image analysis, and assessment of transendothelial migration rates. For complete details on the use and execution of this protocol, please refer to Ladaigue et al..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331580 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102388 | DOI Listing |
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