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http://dx.doi.org/10.1093/pcmedi/pbad013 | DOI Listing |
Nat Med
August 2025
Department of Thoracic Surgery and Oncology, The First Affiliated Hospital of Guangzhou Medical University, National Clinical Research Centre for Respiratory Disease, Guangzhou, China.
Transpl Int
August 2025
Children's Hospital of Los Angeles, University of Southern California, Los Angeles, CA, United States.
Over the past 40 years, the pig-to-nonhuman primate organ transplantation model has enabled progress in xenotransplantation to be made to the point that we are now carrying out initial US FDA-approved clinical experiments on "compassionate" grounds. More recently, the pig-to-human brain-dead decedent model was introduced with claims that this might replace (or at least augment) the pig-to-NHP model. There are, however, several limitations of the decedent model, most notably the very limited period during which the subject may remain sufficiently metabolically and hemodynamically stable to allow meaningful monitoring of the fate of a pig organ graft.
View Article and Find Full Text PDFHepatol Commun
September 2025
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhej
Despite its proven effectiveness in treating liver failure, liver transplantation is often impractical due to its high surgical risks involved and the limited availability of donor organs. Blood purification technologies used in non-bioartificial liver (NBAL) systems have improved clinical cure rates, but they do not substitute for the complex biosynthesis, detoxification, metabolism, and biological transformation roles of the liver. To overcome these challenges brought by NBAL systems, bioartificial liver (BAL) systems have been developed with repeated optimization of both hepatocyte sources and bioreactors to emulate a "fully functional liver".
View Article and Find Full Text PDFNat Med
August 2025
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Recent advancements in genetically engineered pigs have spurred research into pig-to-human xenotransplantation, particularly involving heart, kidney and liver. Here we characterize immune cell populations cells in both peripheral blood and transplanted liver in a human decedent who received a pig liver xenograft and who was monitored over the course of 10 days. Using single-cell RNA sequencing and spatial RNA sequencing, we found that T cells were progressively activated in the peripheral blood, whereas γδT cells and exhausted T cells infiltrated the pig liver extensively, indicating impaired adaptive immunity.
View Article and Find Full Text PDF