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Although various spectroscopic methods have been developed to capture ion-concentration profile changes, it is still difficult to visualize the ion-concentration profile and surface topographical changes simultaneously during the charging/discharging of lithium-ion batteries (LIBs). To tackle this issue, we have developed an scanning ion conductance microscopy (SICM) method that can directly visualize an ion-concentration profile and surface topography using a SICM nanopipette while controlling the sample potential or current with a potentiostat for characterizing the polarization state during charging/discharging. Using SICM on the negative electrode (anode) of LIBs, we have characterized ion-concentration profile changes and the reversible volume changes related to the phase transition during cyclic voltammetry (CV) and charge/discharge of the graphite anode. SICM is a versatile technique that is likely to be of major value for evaluating the correlation between the electrolyte concentration profile and nanoscale surface topography changes.
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http://dx.doi.org/10.1021/jacsau.2c00677 | DOI Listing |
Arch Microbiol
September 2025
College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000, China.
The esterase gene encoding EstJN1 of Clostridium butyricum, which was isolated from the pit cellar of Chinese liquor facility, was expressed. EstJN1 was identified as a novel GDSL esterase belonging to family II. The enzyme demonstrated a marked substrate preference for p-nitrophenyl butyrate, with optimal activity at a temperature of 40 ℃ and a pH of 7.
View Article and Find Full Text PDFFungal Biol
October 2025
University of Warsaw, Faculty of Biology, Białowieża Geobotanical Station, Sportowa 19, 17-230, Białowieża, Poland. Electronic address:
Wild mushrooms can be an important source of protein and essential amino acids, however very little is known about the environmental factors affecting the content of these compounds. In our study, we investigated the influence of soil properties (soil type, C/N ratio, pH) and tree stand characteristics (tree diversity, canopy cover, understory cover, and the proportion of deciduous trees) on total protein and essential amino acids (Valine, Leucine, Isoleucine, Phenylalanine, Lysine, Methionine, Arginine, Histidine) contents in seven wild-growing mushroom species (Macrolepiota procera, Rhodocolybia butyracea, Russula cyanoxantha, R.heterophylla, Lactifluus vellereus, Armillaria mellea s.
View Article and Find Full Text PDFMAbs
December 2025
Antibody Discovery & Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany.
The discovery and development of multispecific antibodies present unique challenges in optimizing their physicochemical properties to enhance developability and manufacturability. Common developability challenges include increased risk of aggregation, high viscosity, poor solubility, low expression yields, complex purification requirements, greater propensity for fragmentation, immunogenicity, or pharmacokinetics. In this study, we systematically investigate the solution behavior of engineered bispecific IgG1-VHH constructs derived from a parental NKp30 ×EGFR natural killer cell engager (NKCE) molecule, focusing on colloidal stability, hydrophobicity, thermal stability, pH sensitivity, and viscosity.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Department of Agricultural and Biosystems Engineering, Faculty of Agriculture, Benha University, P.O. Box 13736, Moshtohor, Qaluobia, Egypt.
The physicochemical, functional, rheological, conformational, and thermal attributes of cottonseed meal protein (CMP) prepared by pH-shifting and/or ultrasonication were investigated. CMP isolates were altered using acidic (pH 1.5, 3.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Post-Graduate Program in Food Science and Technology, Department of Food Engineering, Technology Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
This study evaluated the impact of different concentrations of anthocyanin extract (CAE) from Catharanthus roseus flowers, an innovative and little-explored source of anthocyanins, on the properties of intelligent films for monitoring the freshness of tilapia fillets. The films were produced from collagen from tilapia skin and pectin, in 4 treatments: FC (control), F10 (10 % CAE), F20 (20 % CAE) and F30 (30 % CAE). The results demonstrated that CAE presented high anthocyanin content, significant antioxidant activity, and phenolic profile composed of phenolic acids, flavonoids and anthocyanins, in addition to color variation sensitive to changes in pH.
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