Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Ever since the catalytic hairpin assembly (CHA) circuit has been highlighted as a powerful nucleic acid detection tool, nucleic acid detection methods based on CHA have been widely studied. However, the detection sensitivity of CHA-based methods is insufficient. The relatively high background signals resulting from the spontaneous reaction between the two hairpin probes is one of the major reasons for limiting the sensitivity of CHA. In this study, we established that the addition of deoxynucleotide triphosphates (dNTPs) to the reaction system can significantly reduce the background leakage of CHA. The dNTPs-CHA, coupled with a fluorescence lateral flow assay strip, is used for the rapid and highly sensitive detection of miRNA. It is capable of reliably detecting miRNA in serum samples down to a limit of 100 aM, which is an improvement in the lower detection limit by nearly five orders of magnitude compared to that of the pure CHA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074145 | PMC |
| http://dx.doi.org/10.1016/j.isci.2023.106390 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nitrous oxide (N2O) recreational use is increasing across Germany - a survey of the German neurological society among practicing neurologists.
Neurol Res Pract
September 2025
German Neurological Society, Berlin, Germany.
Background: Recreational nitrous oxide (NO) abuse has become increasingly prevalent, raising concerns about associated health risks. In Germany, the lack of reliable data on NO consumption patterns limits the development of effective public health interventions. This study aims to address this knowledge gap by examining trends, determinants, and health consequences of NO abuse in Germany.
View Article and Find Full Text PDFConstruction of an Ag-functionalized structural color hydrogel sensor for colorimetric detection of glutathione.
Mikrochim Acta
September 2025
Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
An Ag-functionalized structural color hydrogel (Ag-SCH) sensor is constructed for colorimetric detection of glutathione (GSH). The hydrogel is prepared by using the coordination of Ag and 1-vinylimidazole (1-VI) as cross-linking network. GSH acts as a competitive ligand to break the coordination between Ag and 1-VI, leading to the expansion and structural color change of the hydrogel.
View Article and Find Full Text PDFPrevalence of Liver Dysfunction After One-Anastomosis Gastric Bypass: A Systematic Review and Single-Arm Meta-analysis.
Obes Surg
September 2025
St Vincent's Hospital Sydney, Darlinghurst, Australia.
Background: One-anastomosis gastric bypass (OAGB) has gained popularity as a bariatric operation due to its shorter operation time and lower perioperative complication rates, compared with Roux-en-Y gastric bypass (RYGB). However, OAGB is associated with short and long-term complications. Notably, in some reports a subset of patients developed liver dysfunction after OAGB, in some cases causing death or requiring liver transplantation.
View Article and Find Full Text PDFHybrid ZrO₂@CNTs nanocomposite electrodes for ultra-sensitive glucose detection via electrochemical biosensing.
Mikrochim Acta
September 2025
Faculty of Science, Shenyang University of Chemical Technology, Shenyang, 110142, China.
A sensitive electrochemical glucose biosensor using ZrO₂@CNTs nanocomposite was developed for real-time metabolism monitoring for athletes. The nanocomposite was prepared by a simple ultrasound-assisted technique, and the glucose oxidase (GOx) was covalently immobilized to improve the biorecognition ability. CNTs treated with acid served as a highly conductive framework, and ZrO₂ nanoparticles can provide structural stability and catalytic performance, thus showing synergistic enhancement of electron transfer kinetics and enzyme loading capacity.
View Article and Find Full Text PDFReal-World Evaluation of AI-Driven Diabetic Retinopathy Screening in Public Health Settings: Validation and Implementation Study.
JMIR Med Inform
September 2025
Global Health Economics Centre, Public Health and Policy, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Background: Artificial intelligence (AI) algorithms offer an effective solution to alleviate the burden of diabetic retinopathy (DR) screening in public health settings. However, there are challenges in translating diagnostic performance and its application when deployed in real-world conditions.
Objective: This study aimed to assess the technical feasibility of integration and diagnostic performance of validated DR screening (DRS) AI algorithms in real-world outpatient public health settings.