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Article Abstract
Background: Nitrate is the primary type of nitrogen available to plants, which is absorbed and transported by nitrate transporter 2 (NRT2) at low nitrate conditions.
Methods: Genome-wide identification of genes in was performed. Gene expression patterns were revealed using RNA-seq and qRT-PCR. Gene functions were characterized using overexpression in and silencing in . Protein interactions were verified by yeast two-hybrid and luciferase complementation imaging (LCI) assays.
Results: We identified 14, 14, seven, and seven proteins in , , , and . Most NRT2 proteins were predicted in the plasma membrane. The genes were classified into four distinct groups through evolutionary relationships, with members of the same group similar in conserved motifs and gene structure. The promoter regions of genes included many elements related to growth regulation, phytohormones, and abiotic stresses. Tissue expression pattern results revealed that most genes were specifically expressed in roots. Under low nitrate conditions, genes exhibited different expression levels, with being the most up-regulated. plants overexpressing exhibited increased biomass, nitrogen and nitrate accumulation, nitrogen uptake and utilization efficiency, nitrogen-metabolizing enzyme activity, and amino acid content under low nitrate conditions. In addition, -silenced plants exhibited suppressed nitrate uptake and accumulation, hampered plant growth, affected nitrogen metabolism processes, and reduced tolerance to low nitrate. The results showed that could promote nitrate uptake and transport under low nitrate conditions, thus effectively increasing nitrogen use efficiency (NUE). We found that GhNRT2.1e interacts with GhNAR2.1 by yeast two-hybrid and LCI assays.
Discussion: Our research lays the foundation to increase NUE and cultivate new cotton varieties with efficient nitrogen use.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064996 | PMC |
| http://dx.doi.org/10.7717/peerj.15152 | DOI Listing |
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