Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d2cc04366a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quantification of Trans- and Cis-Vitamin K1 Isomers in Human Plasma by a Rapid and Sensitive LC-MS/MS Method With Surrogate Matrix.
Biomed Chromatogr
October 2025
Department of Pharmaceutical Analysis, Pharmacy School, Shenyang Pharmaceutical University, Shenyang, China.
A rapid and specific liquid chromatography-tandem mass spectrometry method with a wide linear range was developed and validated for the simultaneous quantification of Vitamin K1 (VK1) trans- and cis- isomers in human plasma. Bovine serum albumin solution (15%) served as a surrogate matrix for preparing the calibrators to establish the quantitative curves. After liquid-liquid extraction, VK1 trans- and cis- isomers in plasma samples were separated on a ChromCore C30 column (15 cm × 4.
View Article and Find Full Text PDFMitochondria-associated condensates maintain mitochondrial homeostasis and promote lifespan.
Nat Aging
September 2025
State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan and Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming, China.
Membraneless organelles assembled by liquid-liquid phase separation interact with diverse membranous organelles to regulate distinct cellular processes. It remains unknown how membraneless organelles are engaged in mitochondrial homeostasis. Here we demonstrate that mitochondria-associated translation organelles (MATOs) mediate local synthesis of proteins required for structural and functional maintenance of mitochondria.
View Article and Find Full Text PDFCorrection: Liquid-liquid phase separation of GPS2-LATS1 promotes colorectal cancer progression by reprogramming lipid metabolism.
Oncogene
September 2025
Laboratory of Molecular Target Therapy of Cancer, Institute of Basic Medical Sciences, Hubei University of Medicine, Shiyan, Hubei, China.
CpG-A induces liquid-liquid phase separation of HMGB1 to activate the RAGE-mediated inflammatory pathway.
Proc Natl Acad Sci U S A
September 2025
State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
High-mobility group box protein 1 (HMGB1) is a chromatin-associated nonhistone protein widely distributed in the nucleus of eukaryotic cells. It is transported extracellularly as a proinflammatory mediator or late warning protein to induce immune and inflammatory reactions upon stimuli such as microbial infection. Here, we have found that HMGB1 directly interacts with bacterial DNA analogue CpG-A in the extracellular environment to undergo liquid-liquid phase separation (LLPS) via its positively charged DNA-binding domain.
View Article and Find Full Text PDFExtraction and determination of esteric compounds in wound disinfectants.
RSC Adv
September 2025
Food and Drug Safety Research Center, Pharmaceutical Sciences Institute, Tabriz University of Medical Sciences Tabriz Iran.
This study focuses on developing an analytical method to efficiently extract and concentrate several adipate and phthalate plasticizers that can migrate from plastic packaging into various wound disinfectants. The study employed an approach that combined dispersive micro solid phase extraction with dispersive liquid-liquid microextraction using ZIF-4 as an adsorbent. The adsorbent was thoroughly characterized to understand its properties.
View Article and Find Full Text PDF