Morphological and Molecular Characterization of Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA.

J Nematol

Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD 20705, USA.

Published: February 2022


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Article Abstract

In August of 2021, several cysts with juveniles and eggs were discovered during a vegetation survey conducted at the Arlington National Cemetery, Virginia. Eight soil samples were collected from the rhizosphere region of the common grass () and processed at the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL). Cysts were light to dark brown in color, and oval to pear-shaped without bullae in young cysts but present in older cysts and with prominent vulval cone. The juveniles had slightly concave stylet knobs projecting sometimes anteriorly, tail tapering gradually to a narrowly rounded terminus, and hyaline tail terminus conspicuous at least twice the length of stylet. The molecular analysis included the analysis of three gene sequence fragments: D2-D3 of 28S rRNA, ITS rRNA, and COI. The nematode species was identified by both morphological and molecular means as Stone's cyst nematode, . Detection of in Virginia represents a new record of this species in the United States, and a second report after Canada in North America.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260822PMC
http://dx.doi.org/10.2478/jofnem-2022-0008DOI Listing

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