Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
() is a common foodborne pathogen that causes fever, vomiting, and other intestinal symptoms, and seriously affects human health and social safety. As a result, a reliable and sensitive detection technique for must be developed. In this work, we proposed a sandwich assay on vancomycin functionalized magnetic beads (Van-MNPs) for detection based on the specific binding between IgG and targets. The Van-MNPs were used as a tool for the separation of target bacteria. The biotin-modified IgG mediates binding between DNA nanoflowers (DNFs) and the target bacteria via interacting with streptavidin. The DNFs prepared by rolling circle amplification (RCA) were employed as a nano-container to enhance the capacity of biotins, and the streptavidin-horseradish peroxidase (SA-HRP) was loaded onto DNFs to catalyze the color change of TMB. Therefore, a colorimetric biosensor based on magnetic separation and rolling circle amplification was developed. The proposed methods for detection showed a limit of detection (LOD) of 3.3 × 10 CFU/mL and excellent specificity. The biosensor has a certain reference value for the detection of in juice.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265873 | PMC |
| http://dx.doi.org/10.3390/foods11131852 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background-Free Rolling Circle Amplification for SERS Bioassay Using a Chimeric Hairpin-Integrated CRISPR/Cas12a System.
Anal Chem
September 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361
Rolling circle amplification (RCA) has revolutionized nucleic acid detection owing to its isothermal simplicity. However, over two decades of clinical application have been hampered by off-target amplification and incompatibility with double-stranded DNA (dsDNA). Herein, a strategy, specifically cleavage of rationally designed DNA/RNA chimeric hairpin preprimer by dsDNA-targeted CRISPR/Cas12a to rlease ssRNA for initiating RCA (SCOPE-RCA), is proposed for nucleic acid identification of African swine fever virus (ASFV).
View Article and Find Full Text PDFRolling circle amplification for next-generation molecular diagnostics, genome analysis, and spatial transcriptome profiling.
Nanoscale
September 2025
Department of Bioengineering & Nano-Bioengineering, Research Center for Bio Materials and Process Development, Incheon National University, Incheon 22012, Republic of Korea.
Rolling circle amplification (RCA) has emerged as a highly versatile and robust isothermal amplification technology, offering exceptional sensitivity, specificity, and scalability for next-generation molecular diagnostics and multi-omics research. Its ability to generate long, repetitive DNA sequences with high fidelity has made it a pivotal tool in disease diagnostics, genomic analysis, and spatial transcriptome profiling. Recent advancements have expanded RCA into various formats, including solution-phase, solid-phase, hydrogel-based, and digital RCA, enhancing its analytical performance and adaptability across diverse biological applications.
View Article and Find Full Text PDFReplication competition drives the selective mtDNA inheritance in Drosophila ovary.
Cell Rep
September 2025
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:
Purifying selection that limits the transmission of harmful mitochondrial DNA (mtDNA) mutations has been observed in both human and animal models. Yet, the precise mechanism underlying this process remains undefined. Here, we present a highly specific and efficient in situ imaging method capable of visualizing mtDNA variants that differ by only a few nucleotides at single-molecule resolution in Drosophila ovaries.
View Article and Find Full Text PDFCircular Rep-encoding single-stranded DNA (CRESS-DNA) virus Rep proteins are multidomain enzymes that mediate viral DNA rolling-circle replication. Reps nick viral DNA to expose a 3' end for polymerase extension, provide an NTP-dependent helicase activity for DNA unwinding, and join nicked ends to form circular viral genomes. Here, we present the first structures of a Rep protein from the family, a newly discovered family of human-associated CRESS-DNA viruses that replicates within the oral protozoan .
View Article and Find Full Text PDFMicrofluidic detection based on size-encoded microbeads and rolling circle amplification for multiplex respiratory virus nucleic acid detection.
Analyst
September 2025
Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
Acute respiratory tract diseases (ARDs) are predominantly caused by viral infections, with one of their hallmark characteristics being multiple viral co-infections. Such multiple viral infections not only complicate therapeutic interventions but also lead to an increase in mortality rates. Most traditional bioassays, however, are limited to identifying a single type of virus, leading to missed diagnoses in samples with multiple respiratory pathogens.
View Article and Find Full Text PDF