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Article Abstract
Background: Ponciri Fructus, a crude drug consisting of the dried immature fruits of (L.) Raf., is a popular folk medicine used for the treatment of allergy and gastrointestinal disorders in Korea and China. In this study, the anti-adipogenic activity of extracts and isolated compounds were evaluated using 3T3-L1 preadipocytes.
Methods: Dried immature fruits were extracted and fractionated into -hexane, ethyl acetate (EtOAc), -butanol and water-soluble fractions. The ethanol extract and fractions were tested for anti-adipogenic activity in the 3T3-L1 cell line. The active fractions (-hexane and EtOAc fractions) were further subjected to chromatographic techniques to isolate and identify active compounds. Furthermore, the isolated compounds were evaluated for their anti-adipogenic activity.
Results: Altogether, seven compounds, including two flavonoids, one phytosteroid and four coumarin derivatives, were isolated. Ethanol extract, -hexane fraction, EtOAc fraction and three isolated compounds (phellopterin, oxypeucedanin and poncirin) showed significant anti-adipogenic activity as observed by reduced lipid deposition in differentiated 3T3-L1 cells. Further, oxypeucedanin downregulated the key adipogenic markers, such as peroxisome proliferator-activated receptors proteins (PPAR-), sterol response element binding proteins-1 (SREBP-1), CCAAT/enhancer binding proteins- (C/EBP-), adipocyte-specific lipid binding proteins (FABP-4), adipocyte fatty acid binding proteins (aP2), lipoprotein lipase (LPL) and leptin.
Conclusion: This study indicated that the ethanol extract, hexane fraction and ethyl acetate fraction of fruits possess strong anti-adipogenic activity, containing the active compounds such as phellopterin, oxypeucedanin and poncirin. Further research is recommended to explore their efficacy and safety in animal and clinical models.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839251 | PMC |
| http://dx.doi.org/10.3390/molecules27030676 | DOI Listing |
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