Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Although the optimal therapy for myocardial infarction includes reperfusion to restore blood flow to the ischemic area, myocardial injury after ischemia/reperfusion usually leads to an inflammatory response, oxidative stress, and cardiomyocyte apoptosis. In this study, rat adipose-derived stem cells were differentiated into low-thermogenic beige adipocytes (LBACs) and high-thermogenic beige adipocytes (HBACs) to study the different cardioprotective effects of heterogeneous expression of brown adipocytes. We found that antioxidant and antiapoptotic factors in H9c2 cardiomyocytes were upregulated by high levels of secreted FGF21 in HBAC conditioned medium (HBAC-CM), whereas FGF21 in HBAC-CM did not affect antioxidative or antiapoptotic cell death in H9c2 cardiomyocytes with knockdown. These results show that NRF2 mediates antioxidative and antiapoptotic effects through the HBAC-secreted factor FGF21. Consistent with this finding, the expression of antioxidant and antiapoptotic genes was upregulated by highly secreted FGF21 after HBAC-CM treatment compared to LBAC-CM treatment in H9c2 cardiomyocytes via NRF2 activation. Furthermore, HBAC-CM significantly attenuated ischemic rat heart tissue injury via NRF2 activation. Based on these findings, we propose that HBAC-CM exerts beneficial effects in rat cardiac ischemia/reperfusion injury by modulating NRF2 and has potential as a promising therapeutic agent for myocardial infarction.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833946 | PMC |
| http://dx.doi.org/10.3390/cells11030567 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stachydrine Alleviates Sepsis-Induced Cardiomyopathy by Inhibiting Ferroptosis via Regulating SIRT1/GPX4 Pathway.
Dose Response
September 2025
Department of Interventional Radiology, The Second People's Hospital of Nantong, Nantong, Jiangsu Province, China.
Objectives: This study investigated the cardioprotective effects of stachydrine (STA) in lipopolysaccharide (LPS)-induced septic mice and H9c2 cardiomyocytes, focusing on its anti-apoptotic, anti-inflammatory, and anti-ferroptotic actions.
Methods: We established an LPS-induced sepsis model in mice and an LPS-stimulated H9c2 cardiomyocyte model in vitro.
Results: STA markedly reduced LPS-induced myocardial apoptosis, as demonstrated by decreased TUNEL-positive cells, and attenuated the elevation of serum cardiac injury markers, including creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), brain natriuretic peptide (BNP), cardiac troponin I (cTnI), and cardiac troponin T (cTnT) levels.
Fxyd5 downregulation protects the heart from ischemia/reperfusion injury by suppressing myocardial inflammation.
Transpl Immunol
September 2025
Department of Cardiovascular Medicine, Tianjin Medical University General Hospital, Tianjin City 300000, PR China. Electronic address:
Background: Myocardial ischemia/reperfusion (I/R) injury is a common cause of death. FXYD domain-containing ion transport regulator-5 (Fxyd5) is a type I membrane protein that plays a significant role in mediating cellular functions. However, the expression and function of Fxyd5 in myocardial I/R injury remain unclear.
View Article and Find Full Text PDF[Three new chalcone C-glycosides from Carthami Flos].
Zhongguo Zhong Yao Za Zhi
July 2025
School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Modern Research Center for Traditional Chinese Medicine, Beijing Institute of Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China.
The chemical components of Carthami Flos were investigated by using macroporous resin, silica gel column chromatography, reversed-phase octadecylsilane(ODS) column chromatography, Sephadex LH-20, and semi-preparative high-performance liquid chromatography(HPLC). The planar structures of the compounds were established based on their physicochemical properties and ultraviolet-visible(UV-Vis), infrared(IR), high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), and nuclear magnetic resonance(NMR) spectroscopic technology. The absolute configurations were determined by comparing the calculated and experimental electronic circular dichroism(ECD).
View Article and Find Full Text PDFNon-anticoagulant heparin alleviates myocardial ischemia-reperfusion injury through inhibiting GSDMD-mediated cardiomyocyte pyroptosis via the HMGB1/RAGE pathway.
Int Immunopharmacol
September 2025
Department of Cardiology, Yueyang Central Hospital, Yueyang, Hunan Province 414000, China. Electronic address:
Background: Heparin reduces myocardial ischemia-reperfusion (I/R) injury, which is associated with pyroptosis. As a derivative of heparin, non-anticoagulant heparin (NAH) is rarely researched in this field. This study aims to explore the mechanisms of NAH in myocardial I/R injury and pyroptosis.
View Article and Find Full Text PDFCircCOL3A1 ameliorates myocardial ischemia-reperfusion injury by regulating miR-29b-3p/MDM2-mediated apoptosis.
Am J Med Sci
August 2025
Heart Center, The Fifth Affiliated Hospital of Xinjiang Medical University, Xinjiang 830011, China. Electronic address:
Background: Cardiovascular diseases (CVDs) are leading causes of mortality globally, with myocardial ischemia-reperfusion (I/R) injury being a critical challenge in clinical settings. Circular RNAs (circRNAs) have emerged as significant molecular players in various pathophysiological conditions, including myocardial I/R injury.
Objective: This study aimed to investigate the role of circCOL3A1 in myocardial I/R injury and its potential regulatory mechanisms involving miR-29b-3p and MDM2.