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Article Abstract
Cyanobacteria release 2-methylisoborneol (MIB) as a secondary metabolite. Here, we propose a reverse transcription quantitative real-time PCR (RT-qPCR) based method to evaluate the MIB-producing potential in source water by detecting the MIB-synthesis gene (mic). A MIBQSF/R primer set was designed based on 35 mic gene sequences obtained from 12 pure-cultured MIB-producing strains and 23 sequences from the NCBI database. This primer set successfully identified all known 43 MIB-producing cyanobacterial strains (12 from this study and 31 from the NCBI database), belonging to different genera, showing a wider coverage than previous primer sets. The efficiency of the method was proved by the amplification efficiency (E = 91.23%), R of the standard curve (0.999), the limit of detection (LOD, 5.7 fg μL), and the limit of quantification (LOQ, 1.86 × 10 gene copies μL). Further, the method was verified by the correlation between the mic gene abundance and MIB concentration 50 field samples from different reservoirs (R = 0.614, p < 0.001) and one reservoir (R = 0.752, p < 0.001), suggesting its potential as an alternative warning tool to evaluate the risk of MIB problems in source water.
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| http://dx.doi.org/10.1016/j.envres.2021.112308 | DOI Listing |
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Article Synopsis
- Cyanobacteria can adjust their photosynthetic pigments to sense and adapt to different colors of light, allowing them to thrive in varying environments.
- Filamentous cyanobacteria, such as Pseudanabaena, produce 2-methylisoborneol (MIB), which contributes to odor problems in water reservoirs, especially in green light where they increase phycoerythrin (PE) levels.
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Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs.
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