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Article Abstract
Glutathione peroxidase 1 (Gpx1) and peroxiredoxin 2 (Prdx2) belong to the thiol peroxidase family of antioxidants, and have been studied for their antioxidant functions and roles in cancers. However, the physiological significance of Gpx1 and Prdx2 during vertebrate embryogenesis are lacking. Currently, we investigated the functional roles of Gpx1 and Prdx2 during vertebrate embryogenesis using as a vertebrate model. Our investigations revealed the zygotic nature of having its localization in the eye region of developing embryos, whereas exhibited a maternal nature and were localized in embryonic ventral blood islands. Furthermore, the -morphants exhibited malformed eyes with incompletely detached lenses. However, the depletion of has not established its involvement with embryogenesis. A molecular analysis of -depleted embryos revealed the perturbed expression of a -lens-specific marker and also exhibited reactive oxygen species (ROS) accumulation in the eye regions of -morphants. Additionally, transcriptomics analysis of -knockout embryos demonstrated the involvement of Wnt, cadherin, and integrin signaling pathways in the development of malformed eyes. Conclusively, our findings indicate the association of with a complex network of embryonic developmental pathways and ROS responses, but detailed investigation is a prerequisite in order to pinpoint the mechanistic details of these interactions.
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| http://dx.doi.org/10.3390/antiox10101636 | DOI Listing |
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