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Article Abstract
Objective: To evaluate multidrug resistant loop-mediated isothermal amplification (MDR-LAMP) assay for the early diagnosis of multidrug-resistant tuberculosis and to compare the mutation patterns associated with the , , and genes at the Chinese Center for Disease Control and Prevention.
Methods: MDR-LAMP assay was evaluated using 100 ( ) isolates obtained from the National Reference Laboratory for Tuberculosis in China. Phenotypic resistance to isoniazid and rifampicin and whole-genome sequencing served as reference standards.
Results: The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of MDR-LAMP were 85.5%, 93.6%, 96.7%, and 74.4% for the detection of resistance to isoniazid and rifampicin, respectively, and 80.5%, 92.3%, 98.6%, and 41.4% for the detection of cultured from smear-positive sputum samples, respectively. When DNA sequencing was used as the reference standard, the sensitivity, specificity, PPV, and NPV of MDR-LAMP were 93.1%, 92.3%, 97.2%, and 82.8% for the detection of and gene mutations, respectively, and 89.1%, 88.9%, 93.4%, and 81.1% for the detection of gene mutation, respectively.
Conclusion: MDR-LAMP is a rapid and accessible assay for the laboratory identification of rifampicin and isoniazid resistance of isolates.
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| http://dx.doi.org/10.3967/bes2021.085 | DOI Listing |
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